1uzi: Difference between revisions

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==Overview==

C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and, thereby inactivates Rho A, B and C GTPases in mammalian cells. The, structure of a tetragonal crystal form has been determined by molecular, replacement and refined to 1.89 A resolution. It is very similar to the, apo structures determined previously from two different monoclinic crystal, forms. An objective reassessment of available apo and nucleotide-bound, C3bot1 structures indicates that, contrary to a previous report, the, protein possesses a rigid core formed largely of beta-strands and that the, general flexure that accompanies NAD binding is concentrated in two, peripheral lobes. Tetragonal crystals disintegrate in the presence of NAD, most likely because of disruption of essential crystal contacts.

C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and thereby inactivates Rho A, B and C GTPases in mammalian cells. The structure of a tetragonal crystal form has been determined by molecular replacement and refined to 1.89 A resolution. It is very similar to the apo structures determined previously from two different monoclinic crystal forms. An objective reassessment of available apo and nucleotide-bound C3bot1 structures indicates that, contrary to a previous report, the protein possesses a rigid core formed largely of beta-strands and that the general flexure that accompanies NAD binding is concentrated in two peripheral lobes. Tetragonal crystals disintegrate in the presence of NAD, most likely because of disruption of essential crystal contacts.

==About this Structure==

Line 14:

[[Category: NAD(+) ADP-ribosyltransferase]]

[[Category: Single protein]]

[[Category: Acharya, K.R.]]

[[Category: Acharya, K R.]]

[[Category: Ayriss, J.]]

[[Category: Evans, H.R.]]

[[Category: Evans, H R.]]

[[Category: Holloway, D.E.]]

[[Category: Holloway, D E.]]

[[Category: Shone, C.C.]]

[[Category: Shone, C C.]]

[[Category: Sutton, J.M.]]

[[Category: Sutton, J M.]]

[[Category: GOL]]

[[Category: V4O]]

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[[Category: transferase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:30:02 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and, thereby inactivates Rho A, B and C GTPases in mammalian cells. The, structure of a tetragonal crystal form has been determined by molecular, replacement and refined to 1.89 A resolution. It is very similar to the, apo structures determined previously from two different monoclinic crystal, forms. An objective reassessment of available apo and nucleotide-bound, C3bot1 structures indicates that, contrary to a previous report, the, protein possesses a rigid core formed largely of beta-strands and that the, general flexure that accompanies NAD binding is concentrated in two, peripheral lobes. Tetragonal crystals disintegrate in the presence of NAD, most likely because of disruption of essential crystal contacts.
+C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and thereby inactivates Rho A, B and C GTPases in mammalian cells. The structure of a tetragonal crystal form has been determined by molecular replacement and refined to 1.89 A resolution. It is very similar to the apo structures determined previously from two different monoclinic crystal forms. An objective reassessment of available apo and nucleotide-bound C3bot1 structures indicates that, contrary to a previous report, the protein possesses a rigid core formed largely of beta-strands and that the general flexure that accompanies NAD binding is concentrated in two peripheral lobes. Tetragonal crystals disintegrate in the presence of NAD, most likely because of disruption of essential crystal contacts.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: NAD(+) ADP-ribosyltransferase]]
 [[Category: Single protein]]
-[[Category: Acharya, K.R.]]
+[[Category: Acharya, K R.]]
 [[Category: Ayriss, J.]]
-[[Category: Evans, H.R.]]
+[[Category: Evans, H R.]]
-[[Category: Holloway, D.E.]]
+[[Category: Holloway, D E.]]
-[[Category: Shone, C.C.]]
+[[Category: Shone, C C.]]
-[[Category: Sutton, J.M.]]
+[[Category: Sutton, J M.]]
 [[Category: GOL]]
 [[Category: V4O]]
@@ Line 27: / Line 27: @@
 [[Category: transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:13:02 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:30:02 2008''