1v75: Difference between revisions

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New page: left|200px<br /> <applet load="1v75" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v75, resolution 2.02Å" /> '''Crystal structure o...
 
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[[Image:1v75.gif|left|200px]]<br />
[[Image:1v75.gif|left|200px]]<br /><applet load="1v75" size="350" color="white" frame="true" align="right" spinBox="true"  
<applet load="1v75" size="450" color="white" frame="true" align="right" spinBox="true"  
caption="1v75, resolution 2.02&Aring;" />
caption="1v75, resolution 2.02&Aring;" />
'''Crystal structure of hemoglobin D from the Aldabra giant tortoise (Geochelone gigantea) at 2.0 A resolution'''<br />
'''Crystal structure of hemoglobin D from the Aldabra giant tortoise (Geochelone gigantea) at 2.0 A resolution'''<br />


==Overview==
==Overview==
Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a, precipitant solution containing 10% polyethylene glycol 3350 and 50 mM, HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at, least a 2.0 A resolution, belong to the monoclinic space group C2 with, unit cell dimensions of a = 112.1 A, b = 62.4 A, c = 54.0 A, and beta =, 110.3 degrees. One alphabeta dimer molecule of Hb D existed in an, asymmetric unit, with a calculated value of Vm of 2.77 A(3)Da(-1).
Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 A resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 A, b = 62.4 A, c = 54.0 A, and beta = 110.3 degrees. One alphabeta dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 A(3)Da(-1).


==About this Structure==
==About this Structure==
1V75 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dipsochelys_dussumieri Dipsochelys dussumieri] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V75 OCA].  
1V75 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dipsochelys_dussumieri Dipsochelys dussumieri] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V75 OCA].  


==Reference==
==Reference==
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[[Category: the aldabra giant tortoise]]
[[Category: the aldabra giant tortoise]]


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Revision as of 13:32, 21 February 2008

File:1v75.gif


1v75, resolution 2.02Å

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Crystal structure of hemoglobin D from the Aldabra giant tortoise (Geochelone gigantea) at 2.0 A resolution

Overview

Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 A resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 A, b = 62.4 A, c = 54.0 A, and beta = 110.3 degrees. One alphabeta dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 A(3)Da(-1).

About this Structure

1V75 is a Protein complex structure of sequences from Dipsochelys dussumieri with HEM as ligand. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray diffraction study of hemoglobin D from the Aldabra giant tortoise, Geochelone gigantea., Kuwada T, Hasegawa T, Satoh I, Ishikawa K, Shishikura F, Protein Pept Lett. 2003 Aug;10(4):422-5. PMID:14529497

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