1v96: Difference between revisions
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New page: left|200px<br /><applet load="1v96" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v96, resolution 1.75Å" /> '''Crystal structure of... |
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[[Image:1v96.gif|left|200px]]<br /><applet load="1v96" size=" | [[Image:1v96.gif|left|200px]]<br /><applet load="1v96" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1v96, resolution 1.75Å" /> | caption="1v96, resolution 1.75Å" /> | ||
'''Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3'''<br /> | '''Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3'''<br /> | ||
==Overview== | ==Overview== | ||
The Pyrococcus horikoshii OT3 protein PH0500 is highly conserved within | The Pyrococcus horikoshii OT3 protein PH0500 is highly conserved within the Pyrococcus genus of hyperthermophilic archaea and shows low amino-acid sequence similarity with a family of PIN-domain proteins. The protein has been expressed, purified and crystallized in two crystal forms: PH0500-I and PH0500-II. The structure was determined at 2.0 A by the multiple anomalous dispersion method using a selenomethionyl derivative of crystal form PH0500-I (PH0500-I-Se). The structure of PH0500-I has been refined at 1.75 A resolution to an R factor of 20.9% and the structure of PH0500-II has been refined at 2.0 A resolution to an R factor of 23.4%. In both crystal forms as well as in solution the molecule appears to be a dimer. Searches of the databases for protein-fold similarities confirmed that the PH0500 protein is a PIN-domain protein with possible exonuclease activity and involvement in DNA or RNA editing. | ||
==About this Structure== | ==About this Structure== | ||
1V96 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1V96 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V96 OCA]. | ||
==Reference== | ==Reference== | ||
Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii., Jeyakanthan J, Inagaki E, Kuroishi C, Tahirov TH, Acta | Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii., Jeyakanthan J, Inagaki E, Kuroishi C, Tahirov TH, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):463-8. Epub 2005 Apr 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511069 16511069] | ||
[[Category: Pyrococcus horikoshii]] | [[Category: Pyrococcus horikoshii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Jeyakanthan, J.]] | [[Category: Jeyakanthan, J.]] | ||
[[Category: RSGI, RIKEN | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] | ||
[[Category: Tahirov, T | [[Category: Tahirov, T H.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: nucleotide binding protein]] | [[Category: nucleotide binding protein]] | ||
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[[Category: trna synthetase]] | [[Category: trna synthetase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:32:48 2008'' | ||
