Sandbox 719: Difference between revisions
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===Advantage of the DsRed protein=== | ===Advantage of the DsRed protein=== | ||
In vivo, this protein obviously participate to the natural coloration of its host (in this Aequorea victoria), and it had a role in the protection against UV radiation. In vitro the DsRed can be used complementary to the famou Green flurescent protein GFP. Actually, Dsred absorbs in green at 558 nm and emitting a red fluorescente light at 583 nm. This wavelenght is very higher than all the other fluorescent protein, and allow to detect this red-emmiting easily in cytometry or fluorescent microscopy. Futhermore, in addtion to this use in multicolor tagging axperiments, this protein, and all red-emitting proteins, could be helpful to avoid self-fluorescence of the study design . They could also extand the range of resonance energy in transfert-based experiments (FRET). So in this application, pairs of fluorescent can be uses to detect proximity-related phenomena in vivo. | In vivo, this protein obviously participate to the natural coloration of its host (in this Aequorea victoria), and it had a role in the protection against UV radiation. In vitro the DsRed can be used complementary to the famou Green flurescent protein GFP. Actually, Dsred absorbs in green at 558 nm and emitting a red fluorescente light at 583 nm. This wavelenght is very higher than all the other fluorescent protein, and allow to detect this red-emmiting easily in cytometry or fluorescent microscopy. Futhermore, in addtion to this use in multicolor tagging axperiments, this protein, and all red-emitting proteins, could be helpful to avoid self-fluorescence of the study design . They could also extand the range of resonance energy in transfert-based experiments (FRET). So in this application, pairs of fluorescent can be uses to detect proximity-related phenomena in vivo. | ||
On an other side, DsRed had also several drawbacks for its uses in laboratory : | On an other side, DsRed had also several drawbacks for its uses in laboratory : | ||
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=== Experimental Details of the Crystallization === | === Experimental Details of the Crystallization === | ||
[[Image:1g7k.jpg |600px|right|]] | |||
'''Method''': X-RAY DIFFRACTION | '''Method''': X-RAY DIFFRACTION | ||
