Sandbox Reserved 706: Difference between revisions
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(A) Contact area 1, | (A) Contact area 1, | ||
(C) Contact area 2, | (C) Contact area 2, | ||
(E) Contact area 3.]] | (E) Contact area 3.<ref>PMID:18940604</ref>]] | ||
*The first contact area of the Rab27B/Slac2-a interface consists of <scene name='Sandbox_Reserved_706/6a9_rabcdr/2'> residues 6 to 9 from the Rab complementarity-determining regions (RabCDR)</scene> , part of the switch and interswitch regions of Rab27B, and the coiled-coil regions of Slac2-a.<ref>PMID:18940604</ref> In this first contact area we can find three different types of interactions, electrostatic, hydrophobic and hydrogen bonds: (see figure 4A) | *The first contact area of the Rab27B/Slac2-a interface consists of <scene name='Sandbox_Reserved_706/6a9_rabcdr/2'> residues 6 to 9 from the Rab complementarity-determining regions (RabCDR)</scene> , part of the switch and interswitch regions of Rab27B, and the coiled-coil regions of Slac2-a.<ref>PMID:18940604</ref> In this first contact area we can find three different types of interactions, electrostatic, hydrophobic and hydrogen bonds: (see figure 4A) | ||
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<scene name='Sandbox_Reserved_706/Arg128_ala121gln118/1'>Arg128 of Slac2-a, which is highly conserved among the Slp-family proteins, makes two hydrogen bonds with the carbonyl groups of Gln118 and Ala121 of Rab27B</scene>. | <scene name='Sandbox_Reserved_706/Arg128_ala121gln118/1'>Arg128 of Slac2-a, which is highly conserved among the Slp-family proteins, makes two hydrogen bonds with the carbonyl groups of Gln118 and Ala121 of Rab27B</scene>. | ||
* The third contact area is the smallest one . It involves the <scene name='Sandbox_Reserved_706/Zinc_binding_domain_slac2a/1'>zinc-binding subdomain</scene> of Slac2-a and the <scene name='Sandbox_Reserved_706/Loop_entre_beta_2_et_3/1'>β2–β3 loop</scene> of Rab27B, with superposition of their molecular surfaces. (See figure 4E) | * The third contact area is the smallest one . It involves the <scene name='Sandbox_Reserved_706/Zinc_binding_domain_slac2a/1'>zinc-binding subdomain</scene> of Slac2-a and the <scene name='Sandbox_Reserved_706/Loop_entre_beta_2_et_3/1'>β2–β3 loop</scene> of Rab27B, with superposition of their molecular surfaces.<ref>PMID:18940604</ref> (See figure 4E) | ||
These findings strongly suggested that Tyr6 from RabCDR and three residues | These findings strongly suggested that <scene name='Sandbox_Reserved_706/Leu84_phe88_asp91_tyr6/1'>Tyr6 from RabCDR and three residues, Leu84, Phe88, and Asp91</scene> from switch 2 of Rab27 are the minimum determinants of Slac2-a binding.<ref>PMID:18940604</ref> | ||
== '''External Resources''' == | == '''External Resources''' == | ||
