Sandbox 719: Difference between revisions
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Zoé Blanck (talk | contribs) |
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==== The tetramer Interface ==== | ==== The tetramer Interface ==== | ||
[[Image:Tetramer-interface.jpg | | [[Image:Tetramer-interface.jpg |200px|left|thumb|Ribbon diagram of the DsRed tetramer, produced by MOLSCRIPT (28). Monomers are labeled with uppercase A–D, and the carboxy termini are labeled with lowercase a– d. The amino termini are obscured in this view. Note the antenna-like array of the chromophores, antiparallel in pairs.]] | ||
The most striking feature of DsRed is that, as shown in Fig. 2, it exists as an extremely close-packed tetramer, quite unlike the primarily monomeric avGFP. The tetramer is a square prism with remarkably flat sides and a small elliptical hole directly through the center. The hole is lined with polar residues and salt bridges and localizes a number of solvent molecules. | The most striking feature of DsRed is that, as shown in Fig. 2, it exists as an extremely close-packed tetramer, quite unlike the primarily monomeric avGFP. The tetramer is a square prism with remarkably flat sides and a small elliptical hole directly through the center. The hole is lined with polar residues and salt bridges and localizes a number of solvent molecules. | ||
The accessible surface area of the isolated monomer (21) is 10,230 Å2, and that of the tetramer is 31,420 Å2, so roughly 25% of the monomer surface is not solvent accessible in the tetramer. | The accessible surface area of the isolated monomer (21) is 10,230 Å2, and that of the tetramer is 31,420 Å2, so roughly 25% of the monomer surface is not solvent accessible in the tetramer. | ||
