1w9d: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to | Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors. | ||
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Sinapis alba]] | [[Category: Sinapis alba]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transferred entry: 3 | [[Category: Transferred entry: 3 2.1 147]] | ||
[[Category: Arzt, S.]] | [[Category: Arzt, S.]] | ||
[[Category: Bourderioux, A.]] | [[Category: Bourderioux, A.]] | ||
[[Category: Burmeister, W | [[Category: Burmeister, W P.]] | ||
[[Category: Cottaz, S.]] | [[Category: Cottaz, S.]] | ||
[[Category: Gueyrard, D.]] | [[Category: Gueyrard, D.]] | ||
| Line 36: | Line 36: | ||
[[Category: thiohydroximate]] | [[Category: thiohydroximate]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:41:51 2008'' | ||
Revision as of 13:41, 21 February 2008
|
S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE
Overview
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors.
About this Structure
1W9D is a Single protein structure of sequence from Sinapis alba with NAG, ZN, SO4, SEH and GOL as ligands. Active as Transferred entry: 3.2.1.147, with EC number 3.2.3.1 Known structural/functional Site: AGB. Full crystallographic information is available from OCA.
Reference
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170
Page seeded by OCA on Thu Feb 21 15:41:51 2008
