1wmw: Difference between revisions

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[[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1wmw, resolution 1.55Å" />

'''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''<br />

==Overview==

Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8, was expressed in Escherichia coli, purified to homogeneity and, crystallized both as the recombinant native protein and its, selenomethionine (SeMet) derivative. Well diffracting crystals of these, proteins were obtained belonging to the tetragonal space group P4(1) or, P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were, two homodimers in the asymmetric unit. A native data set was collected to, 1.55 A resolution and a data set suitable for MAD phasing was collected to, 2.40 A resolution on beamline BL40B2 at SPring-8.

Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8.

==About this Structure==

1WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].

1WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].

==Reference==

Line 20:

[[Category: Nishio, K.]]

[[Category: Nodake, Y.]]

[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]

[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]

[[Category: Suto, K.]]

[[Category: ggpp]]

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[[Category: thermus]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:05 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1wmw, resolution 1.55&Aring;" />
 '''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''<br />
 ==Overview==
-Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8, was expressed in Escherichia coli, purified to homogeneity and, crystallized both as the recombinant native protein and its, selenomethionine (SeMet) derivative. Well diffracting crystals of these, proteins were obtained belonging to the tetragonal space group P4(1) or, P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were, two homodimers in the asymmetric unit. A native data set was collected to, 1.55 A resolution and a data set suitable for MAD phasing was collected to, 2.40 A resolution on beamline BL40B2 at SPring-8.
+Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8.
 ==About this Structure==
-WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].
+WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: Nishio, K.]]
 [[Category: Nodake, Y.]]
-[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
+[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Suto, K.]]
 [[Category: ggpp]]
@@ Line 29: / Line 29: @@
 [[Category: thermus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:17:00 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:05 2008''