1wn8: Difference between revisions

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[[Image:1wn8.jpg|left|200px]]<br /><applet load="1wn8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1wn8.jpg|left|200px]]<br /><applet load="1wn8" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1wn8" />

'''NMR Structure of OaNTR'''<br />

==Overview==

The cyclotides are the largest family of naturally occurring circular, proteins. The mechanism by which the termini of these gene-encoded, proteins are linked seamlessly with a peptide bond to form a circular, backbone is unknown. Here we report cyclotide-encoding cDNA sequences from, the plant Viola odorata and compare them with those from an evolutionarily, distinct species, Oldenlandia affinis. Individual members of this, multigene family encode one to three mature cyclotide domains. These, domains are preceded by N-terminal repeat regions (NTRs) that are, conserved within a plant species but not between species. We have, structurally characterized peptides corresponding to these NTRs and show, that, despite them having no sequence homology, they form a structurally, conserved alpha-helical motif. This structural conservation suggests a, vital role for the NTR in the in vivo folding, processing, or, detoxification of cyclotide domains from the precursor protein.

The cyclotides are the largest family of naturally occurring circular proteins. The mechanism by which the termini of these gene-encoded proteins are linked seamlessly with a peptide bond to form a circular backbone is unknown. Here we report cyclotide-encoding cDNA sequences from the plant Viola odorata and compare them with those from an evolutionarily distinct species, Oldenlandia affinis. Individual members of this multigene family encode one to three mature cyclotide domains. These domains are preceded by N-terminal repeat regions (NTRs) that are conserved within a plant species but not between species. We have structurally characterized peptides corresponding to these NTRs and show that, despite them having no sequence homology, they form a structurally conserved alpha-helical motif. This structural conservation suggests a vital role for the NTR in the in vivo folding, processing, or detoxification of cyclotide domains from the precursor protein.

==About this Structure==

1WN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1WN8 OCA].

1WN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WN8 OCA].

==Reference==

Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins., Dutton JL, Renda RF, Waine C, Clark RJ, Daly NL, Jennings CV, Anderson MA, Craik DJ, J Biol Chem. 2004 Nov 5;279(45):46858-67. Epub 2004 Aug 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15328347 15328347]

[[Category: Single protein]]

[[Category: Anderson, M.A.]]

[[Category: Anderson, M A.]]

[[Category: Clark, R.J.]]

[[Category: Clark, R J.]]

[[Category: Craik, D.J.]]

[[Category: Craik, D J.]]

[[Category: Daly, N.L.]]

[[Category: Daly, N L.]]

[[Category: Dutton, J.L.]]

[[Category: Dutton, J L.]]

[[Category: Jennings, C.V.]]

[[Category: Jennings, C V.]]

[[Category: Renda, R.F.]]

[[Category: Renda, R F.]]

[[Category: Waine, C.]]

[[Category: helix]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 05:32:~~35 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1wn8.jpg|left|200px]]<br /><applet load="1wn8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1wn8.jpg|left|200px]]<br /><applet load="1wn8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1wn8" />
 '''NMR Structure of OaNTR'''<br />
 ==Overview==
-The cyclotides are the largest family of naturally occurring circular, proteins. The mechanism by which the termini of these gene-encoded, proteins are linked seamlessly with a peptide bond to form a circular, backbone is unknown. Here we report cyclotide-encoding cDNA sequences from, the plant Viola odorata and compare them with those from an evolutionarily, distinct species, Oldenlandia affinis. Individual members of this, multigene family encode one to three mature cyclotide domains. These, domains are preceded by N-terminal repeat regions (NTRs) that are, conserved within a plant species but not between species. We have, structurally characterized peptides corresponding to these NTRs and show, that, despite them having no sequence homology, they form a structurally, conserved alpha-helical motif. This structural conservation suggests a, vital role for the NTR in the in vivo folding, processing, or, detoxification of cyclotide domains from the precursor protein.
+The cyclotides are the largest family of naturally occurring circular proteins. The mechanism by which the termini of these gene-encoded proteins are linked seamlessly with a peptide bond to form a circular backbone is unknown. Here we report cyclotide-encoding cDNA sequences from the plant Viola odorata and compare them with those from an evolutionarily distinct species, Oldenlandia affinis. Individual members of this multigene family encode one to three mature cyclotide domains. These domains are preceded by N-terminal repeat regions (NTRs) that are conserved within a plant species but not between species. We have structurally characterized peptides corresponding to these NTRs and show that, despite them having no sequence homology, they form a structurally conserved alpha-helical motif. This structural conservation suggests a vital role for the NTR in the in vivo folding, processing, or detoxification of cyclotide domains from the precursor protein.
 ==About this Structure==
-WN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WN8 OCA].
+WN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WN8 OCA].
 ==Reference==
 Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins., Dutton JL, Renda RF, Waine C, Clark RJ, Daly NL, Jennings CV, Anderson MA, Craik DJ, J Biol Chem. 2004 Nov 5;279(45):46858-67. Epub 2004 Aug 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15328347 15328347]
 [[Category: Single protein]]
-[[Category: Anderson, M.A.]]
+[[Category: Anderson, M A.]]
-[[Category: Clark, R.J.]]
+[[Category: Clark, R J.]]
-[[Category: Craik, D.J.]]
+[[Category: Craik, D J.]]
-[[Category: Daly, N.L.]]
+[[Category: Daly, N L.]]
-[[Category: Dutton, J.L.]]
+[[Category: Dutton, J L.]]
-[[Category: Jennings, C.V.]]
+[[Category: Jennings, C V.]]
-[[Category: Renda, R.F.]]
+[[Category: Renda, R F.]]
 [[Category: Waine, C.]]
 [[Category: helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:32:35 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:15 2008''