1wo3: Difference between revisions
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New page: left|200px<br /> <applet load="1wo3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wo3" /> '''Solution structure of Minimal Mutant 1 (MM1... |
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[[Image:1wo3.gif|left|200px]]<br /> | [[Image:1wo3.gif|left|200px]]<br /><applet load="1wo3" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''Solution structure of Minimal Mutant 1 (MM1): Multiple alanine mutant of non-native CHANCE domain'''<br /> | '''Solution structure of Minimal Mutant 1 (MM1): Multiple alanine mutant of non-native CHANCE domain'''<br /> | ||
==Overview== | ==Overview== | ||
Zinc binding motifs have received much attention in the area of protein | Zinc binding motifs have received much attention in the area of protein design. Here, we have tested the suitability of a recently discovered nonnative zinc binding structure as a protein design scaffold. A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in approximately 50% of the sequence. We next attempted to transplant binding faces from chosen bimolecular interactions onto one of these mutants, and many of the resulting "chimeras" were shown to adopt a native-like fold. These results both highlight the robust nature of small zinc binding domains and underscore the complexity of designing functional proteins, even using such small, highly ordered scaffolds as templates. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1WO3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] Full crystallographic information is available from [http:// | 1WO3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO3 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Crossley, M.]] | [[Category: Crossley, M.]] | ||
[[Category: Liew, C | [[Category: Liew, C K.]] | ||
[[Category: Mackay, J | [[Category: Mackay, J P.]] | ||
[[Category: Matthews, J | [[Category: Matthews, J M.]] | ||
[[Category: Sharpe, B | [[Category: Sharpe, B K.]] | ||
[[Category: Wilce, J | [[Category: Wilce, J A.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: protein design]] | [[Category: protein design]] | ||
[[Category: zinc finger]] | [[Category: zinc finger]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:46:28 2008'' | ||
