1xdo: Difference between revisions
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New page: left|200px<br /><applet load="1xdo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xdo, resolution 3.0Å" /> '''Crystal Structure of ... |
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[[Image:1xdo.gif|left|200px]]<br /><applet load="1xdo" size=" | [[Image:1xdo.gif|left|200px]]<br /><applet load="1xdo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1xdo, resolution 3.0Å" /> | caption="1xdo, resolution 3.0Å" /> | ||
'''Crystal Structure of Escherichia coli Polyphosphate Kinase'''<br /> | '''Crystal Structure of Escherichia coli Polyphosphate Kinase'''<br /> | ||
==Overview== | ==Overview== | ||
Polyphosphate (polyP), a linear polymer of hundreds of orthophosphate | Polyphosphate (polyP), a linear polymer of hundreds of orthophosphate residues, exists in all tested cells in nature, from pathogenic bacteria to mammals. In bacteria, polyP has a crucial role in stress responses and stationary-phase survival. Polyphosphate kinase (PPK) is the principal enzyme that catalyses the synthesis of polyP in bacteria. It has been shown that PPK is required for bacterial motility, biofilm formation and the production of virulence factors. PPK inhibitors may thus provide a unique therapeutic opportunity against antibiotic-resistant pathogens. Here, we report crystal structures of full-length Escherichia coli PPK and its complex with AMPPNP (beta-gamma-imidoadenosine 5-phosphate). PPK forms an interlocked dimer, with each 80 kDa monomer containing four structural domains. The PPK active site is located in a tunnel, which contains a unique ATP-binding pocket and may accommodate the translocation of synthesized polyP. The PPK structure has laid the foundation for understanding the initiation of polyP synthesis by PPK. | ||
==About this Structure== | ==About this Structure== | ||
1XDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Polyphosphate_kinase Polyphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.1 2.7.4.1] Full crystallographic information is available from [http:// | 1XDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Polyphosphate_kinase Polyphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.1 2.7.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XDO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Huang, W.]] | [[Category: Huang, W.]] | ||
[[Category: Lee, S | [[Category: Lee, S S.]] | ||
[[Category: Xu, W.]] | [[Category: Xu, W.]] | ||
[[Category: Zhu, Y.]] | [[Category: Zhu, Y.]] | ||
[[Category: e | [[Category: e coli polyphosphate kinase]] | ||
[[Category: polyphosphate kinase]] | [[Category: polyphosphate kinase]] | ||
[[Category: ppk]] | [[Category: ppk]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:53:40 2008'' | ||
