1xe3: Difference between revisions

[[Image:1xe3.jpg|left|200px]]<br /><applet load="1xe3" size="350" color="white" frame="true" align="right" spinBox="true"  

Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since these are recognized as potential targets in antibacterial therapy. Purine nucleoside phosphorylase is a key enzyme in the purine-salvage pathway. The crystal structure of purine nucleoside phosphorylase (DeoD) from B. anthracis has been solved by molecular replacement at 2.24 A resolution and refined to an R factor of 18.4%. This is the first report of a DeoD structure from a Gram-positive bacterium.

1XE3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XE3 OCA].  

Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis., Grenha R, Levdikov VM, Fogg MJ, Blagova EV, Brannigan JA, Wilkinson AJ, Wilson KS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):459-62. Epub 2005 Apr 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511068 16511068]

[[Category: Blagova, E V.]]

[[Category: Brannigan, J A.]]

[[Category: Levdikov, V M.]]

[[Category: SPINE, Structural Proteomics in Europe.]]

[[Category: Wilkinson, A J.]]

[[Category: Wilson, K S.]]

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