1xok: Difference between revisions

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[[Image:1xok.gif|left|200px]]<br /><applet load="1xok" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xok.gif|left|200px]]<br /><applet load="1xok" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xok, resolution 3.00Å" />

'''crystal structure of alfalfa mosaic virus RNA 3'UTR in complex with coat protein N terminal peptide'''<br />

==Overview==

Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat, protein binds to the RNA 3' termini. The crystal structure of an alfalfa, mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and, Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon, interacting. Alternating AUGC residues have opposite orientation, and they, base pair in different adjacent duplexes. Localized RNA backbone reversals, stabilized by arginine-guanine interactions place the adenosines and, guanines in reverse order in the duplex. The results suggest that a, uniform, organized 3' conformation, similar to that found on viral RNAs, with transfer RNA-like ends, may be essential for replication.

Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. The crystal structure of an alfalfa mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon interacting. Alternating AUGC residues have opposite orientation, and they base pair in different adjacent duplexes. Localized RNA backbone reversals stabilized by arginine-guanine interactions place the adenosines and guanines in reverse order in the duplex. The results suggest that a uniform, organized 3' conformation, similar to that found on viral RNAs with transfer RNA-like ends, may be essential for replication.

==About this Structure==

1XOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with BR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XOK OCA].

1XOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BR:'>BR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOK OCA].

==Reference==

Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication., Guogas LM, Filman DJ, Hogle JM, Gehrke L, Science. 2004 Dec 17;306(5704):2108-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15604410 15604410]

[[Category: Single protein]]

[[Category: Filman, D.J.]]

[[Category: Filman, D J.]]

[[Category: Gehrke, L.]]

[[Category: Guogas, L.M.]]

[[Category: Guogas, L M.]]

[[Category: Hogle, J.M.]]

[[Category: Hogle, J M.]]

[[Category: BR]]

[[Category: alpha helix]]

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[[Category: protein-rna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:14:~~21 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:57 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xok.gif|left|200px]]<br /><applet load="1xok" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xok.gif|left|200px]]<br /><applet load="1xok" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xok, resolution 3.00&Aring;" />
 '''crystal structure of alfalfa mosaic virus RNA 3'UTR in complex with coat protein N terminal peptide'''<br />
 ==Overview==
-Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat, protein binds to the RNA 3' termini. The crystal structure of an alfalfa, mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and, Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon, interacting. Alternating AUGC residues have opposite orientation, and they, base pair in different adjacent duplexes. Localized RNA backbone reversals, stabilized by arginine-guanine interactions place the adenosines and, guanines in reverse order in the duplex. The results suggest that a, uniform, organized 3' conformation, similar to that found on viral RNAs, with transfer RNA-like ends, may be essential for replication.
+Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. The crystal structure of an alfalfa mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon interacting. Alternating AUGC residues have opposite orientation, and they base pair in different adjacent duplexes. Localized RNA backbone reversals stabilized by arginine-guanine interactions place the adenosines and guanines in reverse order in the duplex. The results suggest that a uniform, organized 3' conformation, similar to that found on viral RNAs with transfer RNA-like ends, may be essential for replication.
 ==About this Structure==
-XOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with BR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XOK OCA].
+XOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BR:'>BR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOK OCA].
 ==Reference==
 Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication., Guogas LM, Filman DJ, Hogle JM, Gehrke L, Science. 2004 Dec 17;306(5704):2108-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15604410 15604410]
 [[Category: Single protein]]
-[[Category: Filman, D.J.]]
+[[Category: Filman, D J.]]
 [[Category: Gehrke, L.]]
-[[Category: Guogas, L.M.]]
+[[Category: Guogas, L M.]]
-[[Category: Hogle, J.M.]]
+[[Category: Hogle, J M.]]
 [[Category: BR]]
 [[Category: alpha helix]]
@@ Line 21: / Line 21: @@
 [[Category: protein-rna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:14:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:57 2008''