1xs0: Difference between revisions

Line 1:

[[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xs0, resolution 1.58Å" />

'''Structure of the E. coli Ivy protein'''<br />

==Overview==

The complete nucleotide sequences of over 37 microbial and three eukaryote, genomes are already publicly available, and more sequencing is in, progress. Despite this accumulation of data, newly sequenced microbial, genomes continue to reveal up to 50% of functionally uncharacterized, "anonymous" genes. A majority of these anonymous proteins have homologues, in other organisms, whereas the rest exhibit no clear similarity to any, other sequence in the data bases. This set of unique, apparently, species-specific, sequences are referred to as ORFans. The biochemical and, structural analysis of ORFan gene products is of both evolutionary and, functional interest. Here we report the cloning and expression of, Escherichia coli ORFan ykfE gene and the functional characterization of, the encoded protein. Under physiological conditions, the protein is a, homodimer with a strong affinity for C-type lysozyme, as revealed by, co-purification and co-crystallization. Activity measurements and, fluorescence studies demonstrated that the YkfE gene product is a potent, C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly, assigned function, ykfE has now been registered under the new gene name, Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock, center.

The complete nucleotide sequences of over 37 microbial and three eukaryote genomes are already publicly available, and more sequencing is in progress. Despite this accumulation of data, newly sequenced microbial genomes continue to reveal up to 50% of functionally uncharacterized "anonymous" genes. A majority of these anonymous proteins have homologues in other organisms, whereas the rest exhibit no clear similarity to any other sequence in the data bases. This set of unique, apparently species-specific, sequences are referred to as ORFans. The biochemical and structural analysis of ORFan gene products is of both evolutionary and functional interest. Here we report the cloning and expression of Escherichia coli ORFan ykfE gene and the functional characterization of the encoded protein. Under physiological conditions, the protein is a homodimer with a strong affinity for C-type lysozyme, as revealed by co-purification and co-crystallization. Activity measurements and fluorescence studies demonstrated that the YkfE gene product is a potent C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly assigned function, ykfE has now been registered under the new gene name Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock center.

==About this Structure==

1XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA].

1XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA].

==Reference==

Line 15:

[[Category: Abergel, C.]]

[[Category: Byrn, D.]]

[[Category: Claverie, J.M.]]

[[Category: Claverie, J M.]]

[[Category: Lazzaroni, J.C.]]

[[Category: Lazzaroni, J C.]]

[[Category: Monchois, V.]]

[[Category: alpha beta fold]]

[[Category: dimer]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:18:~~27 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:02 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xs0, resolution 1.58&Aring;" />
 '''Structure of the E. coli Ivy protein'''<br />
 ==Overview==
-The complete nucleotide sequences of over 37 microbial and three eukaryote, genomes are already publicly available, and more sequencing is in, progress. Despite this accumulation of data, newly sequenced microbial, genomes continue to reveal up to 50% of functionally uncharacterized, "anonymous" genes. A majority of these anonymous proteins have homologues, in other organisms, whereas the rest exhibit no clear similarity to any, other sequence in the data bases. This set of unique, apparently, species-specific, sequences are referred to as ORFans. The biochemical and, structural analysis of ORFan gene products is of both evolutionary and, functional interest. Here we report the cloning and expression of, Escherichia coli ORFan ykfE gene and the functional characterization of, the encoded protein. Under physiological conditions, the protein is a, homodimer with a strong affinity for C-type lysozyme, as revealed by, co-purification and co-crystallization. Activity measurements and, fluorescence studies demonstrated that the YkfE gene product is a potent, C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly, assigned function, ykfE has now been registered under the new gene name, Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock, center.
+The complete nucleotide sequences of over 37 microbial and three eukaryote genomes are already publicly available, and more sequencing is in progress. Despite this accumulation of data, newly sequenced microbial genomes continue to reveal up to 50% of functionally uncharacterized "anonymous" genes. A majority of these anonymous proteins have homologues in other organisms, whereas the rest exhibit no clear similarity to any other sequence in the data bases. This set of unique, apparently species-specific, sequences are referred to as ORFans. The biochemical and structural analysis of ORFan gene products is of both evolutionary and functional interest. Here we report the cloning and expression of Escherichia coli ORFan ykfE gene and the functional characterization of the encoded protein. Under physiological conditions, the protein is a homodimer with a strong affinity for C-type lysozyme, as revealed by co-purification and co-crystallization. Activity measurements and fluorescence studies demonstrated that the YkfE gene product is a potent C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly assigned function, ykfE has now been registered under the new gene name Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock center.
 ==About this Structure==
-XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA].
+XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Abergel, C.]]
 [[Category: Byrn, D.]]
-[[Category: Claverie, J.M.]]
+[[Category: Claverie, J M.]]
-[[Category: Lazzaroni, J.C.]]
+[[Category: Lazzaroni, J C.]]
 [[Category: Monchois, V.]]
 [[Category: alpha beta fold]]
 [[Category: dimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:18:27 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:02 2008''