1xta: Difference between revisions

Line 1:

[[Image:1xta.gif|left|200px]]<br /><applet load="1xta" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xta.gif|left|200px]]<br /><applet load="1xta" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xta, resolution 1.58Å" />

'''Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)'''<br />

==Overview==

Cysteine-rich secretory proteins (CRISPs) play an important role in the, innate immune system and are transcriptionally regulated by androgens in, several tissues. The proteins are mostly found in the epididymis and, granules of mammals, whilst a number of snake venoms also contain, CRISP-family proteins. The natrin protein from the venom of Naja atra, (Taiwan cobra), which belongs to a family of CRISPs and has a, cysteine-rich C-terminal amino-acid sequence, has been purified using a, three-stage chromatography procedure and crystals suitable for X-ray, analysis have been obtained using the hanging-drop vapour-diffusion, method. X-ray diffraction data were collected to 1.58 A resolution using, synchrotron radiation; the crystals belong to space group C222(1), with, unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two, protein molecules in the asymmetric unit and the Matthews coefficient is, estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of, 47.60%.

Cysteine-rich secretory proteins (CRISPs) play an important role in the innate immune system and are transcriptionally regulated by androgens in several tissues. The proteins are mostly found in the epididymis and granules of mammals, whilst a number of snake venoms also contain CRISP-family proteins. The natrin protein from the venom of Naja atra (Taiwan cobra), which belongs to a family of CRISPs and has a cysteine-rich C-terminal amino-acid sequence, has been purified using a three-stage chromatography procedure and crystals suitable for X-ray analysis have been obtained using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.58 A resolution using synchrotron radiation; the crystals belong to space group C222(1), with unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two protein molecules in the asymmetric unit and the Matthews coefficient is estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of 47.60%.

==About this Structure==

1XTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA].

1XTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA].

==Reference==

Line 13:

[[Category: Naja atra]]

[[Category: Single protein]]

[[Category: Chen, C.J.]]

[[Category: Chen, C J.]]

[[Category: Goh, K.X.]]

[[Category: Goh, K X.]]

[[Category: Huang, W.N.]]

[[Category: Huang, W N.]]

[[Category: Lee, S.C.]]

[[Category: Lee, S C.]]

[[Category: Wang, Y.L.]]

[[Category: Wang, Y L.]]

[[Category: Wu, W.G.]]

[[Category: Wu, W G.]]

[[Category: cobra]]

[[Category: crisp]]

Line 26:

[[Category: serine protease]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:19:~~49 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:25 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xta.gif|left|200px]]<br /><applet load="1xta" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xta.gif|left|200px]]<br /><applet load="1xta" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xta, resolution 1.58&Aring;" />
 '''Crystal Structure of Natrin, a snake venom CRISP from Taiwan cobra (Naja atra)'''<br />
 ==Overview==
-Cysteine-rich secretory proteins (CRISPs) play an important role in the, innate immune system and are transcriptionally regulated by androgens in, several tissues. The proteins are mostly found in the epididymis and, granules of mammals, whilst a number of snake venoms also contain, CRISP-family proteins. The natrin protein from the venom of Naja atra, (Taiwan cobra), which belongs to a family of CRISPs and has a, cysteine-rich C-terminal amino-acid sequence, has been purified using a, three-stage chromatography procedure and crystals suitable for X-ray, analysis have been obtained using the hanging-drop vapour-diffusion, method. X-ray diffraction data were collected to 1.58 A resolution using, synchrotron radiation; the crystals belong to space group C222(1), with, unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two, protein molecules in the asymmetric unit and the Matthews coefficient is, estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of, 47.60%.
+Cysteine-rich secretory proteins (CRISPs) play an important role in the innate immune system and are transcriptionally regulated by androgens in several tissues. The proteins are mostly found in the epididymis and granules of mammals, whilst a number of snake venoms also contain CRISP-family proteins. The natrin protein from the venom of Naja atra (Taiwan cobra), which belongs to a family of CRISPs and has a cysteine-rich C-terminal amino-acid sequence, has been purified using a three-stage chromatography procedure and crystals suitable for X-ray analysis have been obtained using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.58 A resolution using synchrotron radiation; the crystals belong to space group C222(1), with unit-cell parameters a = 59.172, b = 65.038, c = 243.156 A. There are two protein molecules in the asymmetric unit and the Matthews coefficient is estimated to be 2.35 A3 Da(-1), corresponding to a solvent content of 47.60%.
 ==About this Structure==
-XTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA].
+XTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTA OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Naja atra]]
 [[Category: Single protein]]
-[[Category: Chen, C.J.]]
+[[Category: Chen, C J.]]
-[[Category: Goh, K.X.]]
+[[Category: Goh, K X.]]
-[[Category: Huang, W.N.]]
+[[Category: Huang, W N.]]
-[[Category: Lee, S.C.]]
+[[Category: Lee, S C.]]
-[[Category: Wang, Y.L.]]
+[[Category: Wang, Y L.]]
-[[Category: Wu, W.G.]]
+[[Category: Wu, W G.]]
 [[Category: cobra]]
 [[Category: crisp]]
@@ Line 26: / Line 26: @@
 [[Category: serine protease]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:19:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:25 2008''