1xvy: Difference between revisions
New page: left|200px<br /><applet load="1xvy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xvy, resolution 1.74Å" /> '''Crystal Structure of... |
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[[Image:1xvy.gif|left|200px]]<br /><applet load="1xvy" size=" | [[Image:1xvy.gif|left|200px]]<br /><applet load="1xvy" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1xvy, resolution 1.74Å" /> | caption="1xvy, resolution 1.74Å" /> | ||
'''Crystal Structure of iron-free Serratia marcescens SfuA'''<br /> | '''Crystal Structure of iron-free Serratia marcescens SfuA'''<br /> | ||
==Overview== | ==Overview== | ||
The uptake of the element iron is vital for the survival of most | The uptake of the element iron is vital for the survival of most organisms. Numerous pathogenic Gram-negative bacteria utilize a periplasm-to-cytosol ATP-binding cassette transport pathway to transport this essential atom in to the cell. In this study, we investigated the Yersinia enterocolitica (YfuA) and Serratia marcescens (SfuA) iron-binding periplasmic proteins. We have determined the 1.8-angstroms structures of iron-loaded (YfuA) and iron-free (SfuA) forms of this class of proteins. Although the sequence of these proteins varies considerably from the other members of the transferrin structural superfamily, they adopt the same three-dimensional fold. The iron-loaded YfuA structure illustrates the unique nature of this new class of proteins in that they are able to octahedrally coordinate the ferric ion in the absence of a bound anion. The iron-free SfuA structure contains a bound citrate anion in the iron-binding cleft that tethers the N- and C-terminal domains of the apo protein and stabilizes the partially open structure. | ||
==About this Structure== | ==About this Structure== | ||
1XVY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1XVY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Yersinia enterocolitica]] | [[Category: Yersinia enterocolitica]] | ||
[[Category: Dougan, D | [[Category: Dougan, D R.]] | ||
[[Category: McRee, D | [[Category: McRee, D E.]] | ||
[[Category: Schryvers, A | [[Category: Schryvers, A B.]] | ||
[[Category: Shouldice, S | [[Category: Shouldice, S R.]] | ||
[[Category: Tari, L | [[Category: Tari, L W.]] | ||
[[Category: CIT]] | [[Category: CIT]] | ||
[[Category: periplasmic iron binding protein]] | [[Category: periplasmic iron binding protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:59:13 2008'' | ||
Revision as of 13:59, 21 February 2008
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Crystal Structure of iron-free Serratia marcescens SfuA
Overview
The uptake of the element iron is vital for the survival of most organisms. Numerous pathogenic Gram-negative bacteria utilize a periplasm-to-cytosol ATP-binding cassette transport pathway to transport this essential atom in to the cell. In this study, we investigated the Yersinia enterocolitica (YfuA) and Serratia marcescens (SfuA) iron-binding periplasmic proteins. We have determined the 1.8-angstroms structures of iron-loaded (YfuA) and iron-free (SfuA) forms of this class of proteins. Although the sequence of these proteins varies considerably from the other members of the transferrin structural superfamily, they adopt the same three-dimensional fold. The iron-loaded YfuA structure illustrates the unique nature of this new class of proteins in that they are able to octahedrally coordinate the ferric ion in the absence of a bound anion. The iron-free SfuA structure contains a bound citrate anion in the iron-binding cleft that tethers the N- and C-terminal domains of the apo protein and stabilizes the partially open structure.
About this Structure
1XVY is a Single protein structure of sequence from Yersinia enterocolitica with CIT as ligand. Full crystallographic information is available from OCA.
Reference
Novel anion-independent iron coordination by members of a third class of bacterial periplasmic ferric ion-binding proteins., Shouldice SR, McRee DE, Dougan DR, Tari LW, Schryvers AB, J Biol Chem. 2005 Feb 18;280(7):5820-7. Epub 2004 Dec 2. PMID:15576371
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