1ygt: Difference between revisions

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[[Image:1ygt.gif|left|200px]]<br /><applet load="1ygt" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ygt.gif|left|200px]]<br /><applet load="1ygt" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ygt, resolution 1.7Å" />

'''Dynein Light Chain TcTex-1'''<br />

==Overview==

TcTex-1, one of three dynein light chains of the dynein motor complex, has, been implicated in targeting and binding cargoes to cytoplasmic dynein for, retrograde or apical transport. Interactions between TcTex-1 and a diverse, set of proteins such as the dynein intermediate chain, Fyn, DOC2, FIP1, the poliovirus receptor, CD155, and the rhodopsin cytoplasmic tail have, been reported; yet, despite the broad range of targets, a consensus, binding sequence remains uncertain. Consequently, we have solved the, crystal structure of the full-length Drosophila homolog of TcTex-1 to 1.7, A resolution using MAD phasing to gain insight into its function and, target specificity. The structure is homodimeric with a domain swapping of, beta-strand 2 and has a fold similar to the dynein light chain, LC8. Based, on structural alignment, the TcTex-1 and LC8 sequences show no identity, although the root mean square deviation between secondary structural, elements is less than 1.6 A. Moreover, the N terminus, which is equivalent, to beta-strand 1 in LC8, is splayed out and binds to a crystallographic, dimer as an anti-parallel beta-strand at the same position as the neuronal, nitric-oxide synthase peptide in the LC8 complex. Similarity to LC8 and, comparison to the LC8-neuronal nitricoxide synthase complex suggest that, TcTex-1 binds its targets in a similar manner as LC8 and provides insight, to the lack of strict sequence identity among the targets for TcTex-1.

TcTex-1, one of three dynein light chains of the dynein motor complex, has been implicated in targeting and binding cargoes to cytoplasmic dynein for retrograde or apical transport. Interactions between TcTex-1 and a diverse set of proteins such as the dynein intermediate chain, Fyn, DOC2, FIP1, the poliovirus receptor, CD155, and the rhodopsin cytoplasmic tail have been reported; yet, despite the broad range of targets, a consensus binding sequence remains uncertain. Consequently, we have solved the crystal structure of the full-length Drosophila homolog of TcTex-1 to 1.7 A resolution using MAD phasing to gain insight into its function and target specificity. The structure is homodimeric with a domain swapping of beta-strand 2 and has a fold similar to the dynein light chain, LC8. Based on structural alignment, the TcTex-1 and LC8 sequences show no identity, although the root mean square deviation between secondary structural elements is less than 1.6 A. Moreover, the N terminus, which is equivalent to beta-strand 1 in LC8, is splayed out and binds to a crystallographic dimer as an anti-parallel beta-strand at the same position as the neuronal nitric-oxide synthase peptide in the LC8 complex. Similarity to LC8 and comparison to the LC8-neuronal nitricoxide synthase complex suggest that TcTex-1 binds its targets in a similar manner as LC8 and provides insight to the lack of strict sequence identity among the targets for TcTex-1.

==About this Structure==

1YGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1YGT OCA].

1YGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGT OCA].

==Reference==

Line 13:

[[Category: Drosophila melanogaster]]

[[Category: Single protein]]

[[Category: Hendrickson, W.A.]]

[[Category: Hendrickson, W A.]]

[[Category: Williams, J.C.]]

[[Category: Williams, J C.]]

[[Category: Xie, H.]]

[[Category: SO4]]

[[Category: domain swapping]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:05:07 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ygt.gif|left|200px]]<br /><applet load="1ygt" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ygt.gif|left|200px]]<br /><applet load="1ygt" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ygt, resolution 1.7&Aring;" />
 '''Dynein Light Chain TcTex-1'''<br />
 ==Overview==
-TcTex-1, one of three dynein light chains of the dynein motor complex, has, been implicated in targeting and binding cargoes to cytoplasmic dynein for, retrograde or apical transport. Interactions between TcTex-1 and a diverse, set of proteins such as the dynein intermediate chain, Fyn, DOC2, FIP1, the poliovirus receptor, CD155, and the rhodopsin cytoplasmic tail have, been reported; yet, despite the broad range of targets, a consensus, binding sequence remains uncertain. Consequently, we have solved the, crystal structure of the full-length Drosophila homolog of TcTex-1 to 1.7, A resolution using MAD phasing to gain insight into its function and, target specificity. The structure is homodimeric with a domain swapping of, beta-strand 2 and has a fold similar to the dynein light chain, LC8. Based, on structural alignment, the TcTex-1 and LC8 sequences show no identity, although the root mean square deviation between secondary structural, elements is less than 1.6 A. Moreover, the N terminus, which is equivalent, to beta-strand 1 in LC8, is splayed out and binds to a crystallographic, dimer as an anti-parallel beta-strand at the same position as the neuronal, nitric-oxide synthase peptide in the LC8 complex. Similarity to LC8 and, comparison to the LC8-neuronal nitricoxide synthase complex suggest that, TcTex-1 binds its targets in a similar manner as LC8 and provides insight, to the lack of strict sequence identity among the targets for TcTex-1.
+TcTex-1, one of three dynein light chains of the dynein motor complex, has been implicated in targeting and binding cargoes to cytoplasmic dynein for retrograde or apical transport. Interactions between TcTex-1 and a diverse set of proteins such as the dynein intermediate chain, Fyn, DOC2, FIP1, the poliovirus receptor, CD155, and the rhodopsin cytoplasmic tail have been reported; yet, despite the broad range of targets, a consensus binding sequence remains uncertain. Consequently, we have solved the crystal structure of the full-length Drosophila homolog of TcTex-1 to 1.7 A resolution using MAD phasing to gain insight into its function and target specificity. The structure is homodimeric with a domain swapping of beta-strand 2 and has a fold similar to the dynein light chain, LC8. Based on structural alignment, the TcTex-1 and LC8 sequences show no identity, although the root mean square deviation between secondary structural elements is less than 1.6 A. Moreover, the N terminus, which is equivalent to beta-strand 1 in LC8, is splayed out and binds to a crystallographic dimer as an anti-parallel beta-strand at the same position as the neuronal nitric-oxide synthase peptide in the LC8 complex. Similarity to LC8 and comparison to the LC8-neuronal nitricoxide synthase complex suggest that TcTex-1 binds its targets in a similar manner as LC8 and provides insight to the lack of strict sequence identity among the targets for TcTex-1.
 ==About this Structure==
-YGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YGT OCA].
+YGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGT OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Drosophila melanogaster]]
 [[Category: Single protein]]
-[[Category: Hendrickson, W.A.]]
+[[Category: Hendrickson, W A.]]
-[[Category: Williams, J.C.]]
+[[Category: Williams, J C.]]
 [[Category: Xie, H.]]
 [[Category: SO4]]
 [[Category: domain swapping]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:45:48 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:05:07 2008''