1yjm: Difference between revisions
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New page: left|200px<br /><applet load="1yjm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yjm, resolution 2.20Å" /> '''Crystal structure of... |
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[[Image:1yjm.gif|left|200px]]<br /><applet load="1yjm" size=" | [[Image:1yjm.gif|left|200px]]<br /><applet load="1yjm" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1yjm, resolution 2.20Å" /> | caption="1yjm, resolution 2.20Å" /> | ||
'''Crystal structure of the FHA domain of mouse polynucleotide kinase in complex with an XRCC4-derived phosphopeptide.'''<br /> | '''Crystal structure of the FHA domain of mouse polynucleotide kinase in complex with an XRCC4-derived phosphopeptide.'''<br /> | ||
==Overview== | ==Overview== | ||
Mammalian polynucleotide kinase (PNK) is a key component of both the base | Mammalian polynucleotide kinase (PNK) is a key component of both the base excision repair (BER) and nonhomologous end-joining (NHEJ) DNA repair pathways. PNK acts as a 5'-kinase/3'-phosphatase to create 5'-phosphate/3'-hydroxyl termini, which are a necessary prerequisite for ligation during repair. PNK is recruited to repair complexes through interactions between its N-terminal FHA domain and phosphorylated components of either pathway. Here, we describe the crystal structure of intact mammalian PNK and a structure of the PNK FHA bound to a cognate phosphopeptide. The kinase domain has a broad substrate binding pocket, which preferentially recognizes double-stranded substrates with recessed 5' termini. In contrast, the phosphatase domain efficiently dephosphorylates single-stranded 3'-phospho termini as well as double-stranded substrates. The FHA domain is linked to the kinase/phosphatase catalytic domain by a flexible tether, and it exhibits a mode of target selection based on electrostatic complementarity between the binding surface and the phosphothreonine peptide. | ||
==About this Structure== | ==About this Structure== | ||
1YJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] Full crystallographic information is available from [http:// | 1YJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJM OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Polynucleotide 5'-hydroxy-kinase]] | [[Category: Polynucleotide 5'-hydroxy-kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bernstein, N | [[Category: Bernstein, N K.]] | ||
[[Category: Cass, C | [[Category: Cass, C E.]] | ||
[[Category: Cui, D.]] | [[Category: Cui, D.]] | ||
[[Category: Durocher, D.]] | [[Category: Durocher, D.]] | ||
[[Category: Galicia, S.]] | [[Category: Galicia, S.]] | ||
[[Category: Glover, J | [[Category: Glover, J N.M.]] | ||
[[Category: Green, R.]] | [[Category: Green, R.]] | ||
[[Category: Karimi-Busheri, F.]] | [[Category: Karimi-Busheri, F.]] | ||
[[Category: Koch, C | [[Category: Koch, C A.]] | ||
[[Category: Mani, R | [[Category: Mani, R S.]] | ||
[[Category: Rakovszky, M | [[Category: Rakovszky, M L.]] | ||
[[Category: Weinfeld, M.]] | [[Category: Weinfeld, M.]] | ||
[[Category: Williams, R | [[Category: Williams, R S.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: fha domain]] | [[Category: fha domain]] | ||
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[[Category: xrcc4 phosphopeptide]] | [[Category: xrcc4 phosphopeptide]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:00 2008'' | ||
