1yl3: Difference between revisions

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[[Image:1yl3.gif|left|200px]]<br /><applet load="1yl3" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1yl3.gif|left|200px]]<br /><applet load="1yl3" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1yl3, resolution 5.500Å" />

'''Crystal structure of 70S ribosome with thrS operator and tRNAs. Large subunit. The coordinates for the small subunit are in the pdb entry 1YL4.'''<br />

==Overview==

The ribosome of Thermus thermophilus was cocrystallized with initiator, transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a, translational operator. The path of the mRNA was defined at 5.5 angstroms, resolution by comparing it with either the crystal structure of the same, ribosomal complex lacking mRNA or with an unstructured mRNA. A precise, ribosomal environment positions the operator stem-loop structure, perpendicular to the surface of the ribosome on the platform of the 30S, subunit. The binding of the operator and of the initiator tRNA occurs on, the ribosome with an unoccupied tRNA exit site, which is expected for an, initiation complex. The positioning of the regulatory domain of the, operator relative to the ribosome elucidates the molecular mechanism by, which the bound repressor switches off translation. Our data suggest a, general way in which mRNA control elements must be placed on the ribosome, to perform their regulatory task.

The ribosome of Thermus thermophilus was cocrystallized with initiator transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a translational operator. The path of the mRNA was defined at 5.5 angstroms resolution by comparing it with either the crystal structure of the same ribosomal complex lacking mRNA or with an unstructured mRNA. A precise ribosomal environment positions the operator stem-loop structure perpendicular to the surface of the ribosome on the platform of the 30S subunit. The binding of the operator and of the initiator tRNA occurs on the ribosome with an unoccupied tRNA exit site, which is expected for an initiation complex. The positioning of the regulatory domain of the operator relative to the ribosome elucidates the molecular mechanism by which the bound repressor switches off translation. Our data suggest a general way in which mRNA control elements must be placed on the ribosome to perform their regulatory task.

==About this Structure==

1YL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1YL3 OCA].

1YL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YL3 OCA].

==Reference==

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[[Category: translational operator]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 06:~~52:54 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:26 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1yl3.gif|left|200px]]<br /><applet load="1yl3" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1yl3.gif|left|200px]]<br /><applet load="1yl3" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1yl3, resolution 5.500&Aring;" />
 '''Crystal structure of 70S ribosome with thrS operator and tRNAs. Large subunit. The coordinates for the small subunit are in the pdb entry 1YL4.'''<br />
 ==Overview==
-The ribosome of Thermus thermophilus was cocrystallized with initiator, transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a, translational operator. The path of the mRNA was defined at 5.5 angstroms, resolution by comparing it with either the crystal structure of the same, ribosomal complex lacking mRNA or with an unstructured mRNA. A precise, ribosomal environment positions the operator stem-loop structure, perpendicular to the surface of the ribosome on the platform of the 30S, subunit. The binding of the operator and of the initiator tRNA occurs on, the ribosome with an unoccupied tRNA exit site, which is expected for an, initiation complex. The positioning of the regulatory domain of the, operator relative to the ribosome elucidates the molecular mechanism by, which the bound repressor switches off translation. Our data suggest a, general way in which mRNA control elements must be placed on the ribosome, to perform their regulatory task.
+The ribosome of Thermus thermophilus was cocrystallized with initiator transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a translational operator. The path of the mRNA was defined at 5.5 angstroms resolution by comparing it with either the crystal structure of the same ribosomal complex lacking mRNA or with an unstructured mRNA. A precise ribosomal environment positions the operator stem-loop structure perpendicular to the surface of the ribosome on the platform of the 30S subunit. The binding of the operator and of the initiator tRNA occurs on the ribosome with an unoccupied tRNA exit site, which is expected for an initiation complex. The positioning of the regulatory domain of the operator relative to the ribosome elucidates the molecular mechanism by which the bound repressor switches off translation. Our data suggest a general way in which mRNA control elements must be placed on the ribosome to perform their regulatory task.
 ==About this Structure==
-YL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YL3 OCA].
+YL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YL3 OCA].
 ==Reference==
@@ Line 27: / Line 27: @@
 [[Category: translational operator]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:52:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:26 2008''