Group:MUZIC:ALP: Difference between revisions

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On the basis of structural similarities, the ALP subfamily are related to the [http://www.proteopedia.org/wiki/index.php/Group:MUZIC:Enigma_Family Enigma subfamily], possessing [http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=PDZ&BLAST=DUMMY PDZ] and [http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=LIM&BLAST=DUMMY LIM] domains, as well as internal motifs ''viz'' ALP-like motif (AM) and ZASP-like motif (ZM). However, the ALP subfamily differs from the [http://www.proteopedia.org/wiki/index.php/Group:MUZIC:Enigma_Family Enigma subfamily] as each member of the ALP subfamily possess an N-terminal PDZ domain and only '''one''' C-terminal LIM domain.

CLP36 ('''C'''-terminal '''L'''IM '''P'''rotein '''36''' kDa)<ref>PMID:8522188</ref> and RIL ('''R'''eversion-'''I'''nduced '''L'''IM)<ref>PMID:7824279</ref> were the first to be ~~characterized~~. α-Actinin-associated LIM protein (ALP) was later identified as a 39 kDa protein in rat skeletal muscle with a slightly shorter isoform in heart <ref name="r1">PMID:9334352</ref>, which shares a high degree of homology with ~~previously characterized~~ CLP36 and RIL ~~proteins~~<ref>doi:10.1093/jmcb/mjp038</ref>. Mystique is the most recently identified and ~~characterized~~ member of ~~this~~ subfamily.

CLP36 ('''C'''-terminal '''L'''IM '''P'''rotein '''36''' kDa)<ref>PMID:8522188</ref> and RIL ('''R'''eversion-'''I'''nduced '''L'''IM)<ref>PMID:7824279</ref> were the first protein members to be characterised in this subfamily. α-Actinin-associated LIM protein (ALP) was later identified as a 39 kDa protein in rat skeletal muscle with a slightly shorter isoform in heart <ref name="r1">PMID:9334352</ref>, which shares a high degree of homology with the first protein members of the subfamily - CLP36 and RIL <ref>doi:10.1093/jmcb/mjp038</ref>. Mystique is the most recently identified and characterised member of the ALP subfamily.

==Sequence annotation==

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'''ALP''' [http://www.uniprot.org/uniprot/Q53GG5#section_features] '''Mystique''' [http://www.uniprot.org/uniprot/Q96JY6] '''RIL''' [http://www.uniprot.org/uniprot/P50479#section_features] '''hCLIM1'''[http://www.uniprot.org/uniprot/O00151#section_features]]]

Apart from the PDZ/LIM domains, ALP subfamily members possess a conserved 34-amino-acid motif called '''ALP-like motif''' <ref>DOI 10.1100/tsw.2007.232</ref>

Apart from the PDZ/LIM domains, ~~all~~ ALP subfamily members possess a conserved 34-amino-acid motif called '''ALP-like motif''' <ref>DOI 10.1100/tsw.2007.232</ref>

'''ALP''' (PDLIM3: PDZ and LIM domain protein 3) is the 39 kDa, 364 amino-acid protein present in heart and skeletal

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== Structures ==

Molecular structures of the PDZ and/or LIM domains of [http://www.pdb.org/pdb/explore/explore.do?structureId=2PKT PDLIM1 (hCLIM1/CLP36)], [http://www.pdb.org/pdb/results/results.do?qrid=185C6F4B&tabtoshow=Current PDLIM2 (Mystique)], [http://www.pdb.org/pdb/results/results.do?qrid=EDAB5DC2&tabtoshow=Current PDLIM3 (ALP)] and [http://www.pdb.org/pdb/results/results.do?qrid=D3075EE4&tabtoshow=Current PDLIM4 (RIL)] have been solved, in thier apo form, and in complex with the C-terminal extension of their binding partners. These structures present insightful features highlighting their respective interactions and functions.

==Function and Interactions==

Group:MUZIC:ALP: Difference between revisions

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 On the basis of structural similarities, the ALP subfamily are related to the [http://www.proteopedia.org/wiki/index.php/Group:MUZIC:Enigma_Family Enigma subfamily], possessing [http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=PDZ&BLAST=DUMMY PDZ] and [http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=LIM&BLAST=DUMMY LIM] domains, as well as internal motifs ''viz'' ALP-like motif (AM) and ZASP-like motif (ZM). However, the ALP subfamily differs from the [http://www.proteopedia.org/wiki/index.php/Group:MUZIC:Enigma_Family Enigma subfamily] as each member of the ALP subfamily possess an N-terminal PDZ domain and only '''one''' C-terminal LIM domain.
-CLP36 ('''C'''-terminal '''L'''IM '''P'''rotein '''36''' kDa)<ref>PMID:8522188</ref> and RIL ('''R'''eversion-'''I'''nduced '''L'''IM)<ref>PMID:7824279</ref> were the first to be characterized. α-Actinin-associated LIM protein (ALP) was later identified as a 39 kDa protein in rat skeletal muscle with a slightly shorter isoform in heart <ref name="r1">PMID:9334352</ref>, which shares a high degree of homology with previously characterized CLP36 and RIL proteins<ref>doi:10.1093/jmcb/mjp038</ref>. Mystique is the most recently identified and characterized member of this subfamily.
+CLP36 ('''C'''-terminal '''L'''IM '''P'''rotein '''36''' kDa)<ref>PMID:8522188</ref> and RIL ('''R'''eversion-'''I'''nduced '''L'''IM)<ref>PMID:7824279</ref> were the first protein members to be characterised in this subfamily. α-Actinin-associated LIM protein (ALP) was later identified as a 39 kDa protein in rat skeletal muscle with a slightly shorter isoform in heart <ref name="r1">PMID:9334352</ref>, which shares a high degree of homology with the first protein members of the subfamily - CLP36 and RIL <ref>doi:10.1093/jmcb/mjp038</ref>. Mystique is the most recently identified and characterised member of the ALP subfamily.
 ==Sequence annotation==
@@ Line 9: / Line 9: @@
 '''ALP''' [http://www.uniprot.org/uniprot/Q53GG5#section_features] '''Mystique''' [http://www.uniprot.org/uniprot/Q96JY6] '''RIL''' [http://www.uniprot.org/uniprot/P50479#section_features] '''hCLIM1'''[http://www.uniprot.org/uniprot/O00151#section_features]]]
+Apart from the PDZ/LIM domains, ALP subfamily members possess a conserved 34-amino-acid motif called '''ALP-like motif''' <ref>DOI 10.1100/tsw.2007.232</ref>
-Apart from the PDZ/LIM domains, all ALP subfamily members possess a conserved 34-amino-acid motif called '''ALP-like motif''' <ref>DOI 10.1100/tsw.2007.232</ref>
 '''ALP''' (PDLIM3: PDZ and LIM domain protein 3) is the 39 kDa, 364 amino-acid protein present in heart and skeletal
@@ Line 23: / Line 21: @@
 == Structures ==
+Molecular structures of the PDZ and/or LIM domains of [http://www.pdb.org/pdb/explore/explore.do?structureId=2PKT PDLIM1 (hCLIM1/CLP36)], [http://www.pdb.org/pdb/results/results.do?qrid=185C6F4B&tabtoshow=Current PDLIM2 (Mystique)], [http://www.pdb.org/pdb/results/results.do?qrid=EDAB5DC2&tabtoshow=Current PDLIM3 (ALP)] and [http://www.pdb.org/pdb/results/results.do?qrid=D3075EE4&tabtoshow=Current PDLIM4 (RIL)] have been solved, in thier apo form, and in complex with the C-terminal extension of their binding partners. These structures present insightful features highlighting their respective interactions and functions.
 ==Function and Interactions==