1yy6: Difference between revisions
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New page: left|200px<br /> <applet load="1yy6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yy6, resolution 1.70Å" /> '''The Crystal Strucur... |
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[[Image:1yy6.gif|left|200px]]<br /> | [[Image:1yy6.gif|left|200px]]<br /><applet load="1yy6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1yy6" size=" | |||
caption="1yy6, resolution 1.70Å" /> | caption="1yy6, resolution 1.70Å" /> | ||
'''The Crystal Strucure of the N-terminal domain of HAUSP/USP7 complexed with an EBNA1 peptide'''<br /> | '''The Crystal Strucure of the N-terminal domain of HAUSP/USP7 complexed with an EBNA1 peptide'''<br /> | ||
==Overview== | ==Overview== | ||
USP7/HAUSP is a key regulator of p53 and Mdm2 and is targeted by the | USP7/HAUSP is a key regulator of p53 and Mdm2 and is targeted by the Epstein-Barr nuclear antigen 1 (EBNA1) protein of Epstein-Barr virus (EBV). We have determined the crystal structure of the p53 binding domain of USP7 alone and bound to an EBNA1 peptide. This domain is an eight-stranded beta sandwich similar to the TRAF-C domains of TNF-receptor associated factors, although the mode of peptide binding differs significantly from previously observed TRAF-peptide interactions in the sequence (DPGEGPS) and the conformation of the bound peptide. NMR chemical shift analyses of USP7 bound by EBNA1 and p53 indicated that p53 binds the same pocket as EBNA1 but makes less extensive contacts with USP7. Functional studies indicated that EBNA1 binding to USP7 can protect cells from apoptotic challenge by lowering p53 levels. The data provide a structural and conceptual framework for understanding how EBNA1 might contribute to the survival of Epstein-Barr virus-infected cells. | ||
==About this Structure== | ==About this Structure== | ||
1YY6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] Full crystallographic information is available from [http:// | 1YY6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YY6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ubiquitin thiolesterase]] | [[Category: Ubiquitin thiolesterase]] | ||
[[Category: Arrowsmith, C | [[Category: Arrowsmith, C H.]] | ||
[[Category: Edwards, A | [[Category: Edwards, A M.]] | ||
[[Category: Frappier, L.]] | [[Category: Frappier, L.]] | ||
[[Category: Holowaty, M.]] | [[Category: Holowaty, M.]] | ||
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[[Category: traf-domain]] | [[Category: traf-domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:16 2008'' | ||
