1z3h: Difference between revisions

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[[Image:1z3h.gif|left|200px]]<br /><applet load="1z3h" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1z3h.gif|left|200px]]<br /><applet load="1z3h" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1z3h, resolution 3.100Å" />

'''The exportin Cse1 in its cargo-free, cytoplasmic state'''<br />

==Overview==

Cse1 mediates nuclear export of importin alpha, the nuclear localization, signal (NLS) import adaptor. We report the 3.1 A resolution structure of, cargo-free Cse1, representing this HEAT repeat protein in its cytosolic, state. Cse1 is compact, consisting of N- and C-terminal arches that, interact to form a ring. Comparison with the structure of cargo-bound Cse1, shows a major conformational change leading to opening of the structure, upon cargo binding. The largest structural changes occur within a hinge, region centered at HEAT repeat 8. This repeat contains a conserved, insertion that connects the RanGTP and importin alpha contact sites and, that is essential for binding. In the cargo-free state, the RanGTP binding, sites are occluded and the importin alpha sites are distorted. Mutations, that destabilize the N- to C-terminal interaction uncouple importin alpha, and Ran binding, suggesting that the closed conformation prevents, association with importin alpha.

Cse1 mediates nuclear export of importin alpha, the nuclear localization signal (NLS) import adaptor. We report the 3.1 A resolution structure of cargo-free Cse1, representing this HEAT repeat protein in its cytosolic state. Cse1 is compact, consisting of N- and C-terminal arches that interact to form a ring. Comparison with the structure of cargo-bound Cse1 shows a major conformational change leading to opening of the structure upon cargo binding. The largest structural changes occur within a hinge region centered at HEAT repeat 8. This repeat contains a conserved insertion that connects the RanGTP and importin alpha contact sites and that is essential for binding. In the cargo-free state, the RanGTP binding sites are occluded and the importin alpha sites are distorted. Mutations that destabilize the N- to C-terminal interaction uncouple importin alpha and Ran binding, suggesting that the closed conformation prevents association with importin alpha.

==About this Structure==

1Z3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA].

1Z3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA].

==Reference==

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[[Category: nuclear transport]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 07:13:~~17 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:11:45 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1z3h.gif|left|200px]]<br /><applet load="1z3h" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1z3h.gif|left|200px]]<br /><applet load="1z3h" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1z3h, resolution 3.100&Aring;" />
 '''The exportin Cse1 in its cargo-free, cytoplasmic state'''<br />
 ==Overview==
-Cse1 mediates nuclear export of importin alpha, the nuclear localization, signal (NLS) import adaptor. We report the 3.1 A resolution structure of, cargo-free Cse1, representing this HEAT repeat protein in its cytosolic, state. Cse1 is compact, consisting of N- and C-terminal arches that, interact to form a ring. Comparison with the structure of cargo-bound Cse1, shows a major conformational change leading to opening of the structure, upon cargo binding. The largest structural changes occur within a hinge, region centered at HEAT repeat 8. This repeat contains a conserved, insertion that connects the RanGTP and importin alpha contact sites and, that is essential for binding. In the cargo-free state, the RanGTP binding, sites are occluded and the importin alpha sites are distorted. Mutations, that destabilize the N- to C-terminal interaction uncouple importin alpha, and Ran binding, suggesting that the closed conformation prevents, association with importin alpha.
+Cse1 mediates nuclear export of importin alpha, the nuclear localization signal (NLS) import adaptor. We report the 3.1 A resolution structure of cargo-free Cse1, representing this HEAT repeat protein in its cytosolic state. Cse1 is compact, consisting of N- and C-terminal arches that interact to form a ring. Comparison with the structure of cargo-bound Cse1 shows a major conformational change leading to opening of the structure upon cargo binding. The largest structural changes occur within a hinge region centered at HEAT repeat 8. This repeat contains a conserved insertion that connects the RanGTP and importin alpha contact sites and that is essential for binding. In the cargo-free state, the RanGTP binding sites are occluded and the importin alpha sites are distorted. Mutations that destabilize the N- to C-terminal interaction uncouple importin alpha and Ran binding, suggesting that the closed conformation prevents association with importin alpha.
 ==About this Structure==
-Z3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA].
+Z3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA].
 ==Reference==
@@ Line 25: / Line 25: @@
 [[Category: nuclear transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:13:17 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:11:45 2008''