1zb6: Difference between revisions

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[[Image:1zb6.gif|left|200px]]<br /><applet load="1zb6" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1zb6.gif|left|200px]]<br /><applet load="1zb6" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1zb6, resolution 1.95Å" />

'''Co-Crystal Structure of ORF2 an Aromatic Prenyl Transferase from Streptomyces sp. strain cl190 complexed with GSPP and 1,6-dihydroxynaphtalene'''<br />

==Overview==

The anti-oxidant naphterpin is a natural product containing a, polyketide-based aromatic core with an attached 10-carbon geranyl group, derived from isoprenoid (terpene) metabolism. Hybrid natural products such, as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl), or 15-carbon (farnesyl) isoprenoid chains possess biological activities, distinct from their non-prenylated aromatic precursors. These hybrid, natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of, aromatic prenyltransferases (PTases) responsible for prenyl group, attachment have only recently been isolated and characterized. Here we, report the gene identification, biochemical characterization and, high-resolution X-ray crystal structures of an architecturally novel, aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates, and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a, 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin, biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and, carbon-oxygen-based prenylation of a diverse collection of, hydroxyl-containing aromatic acceptors of synthetic, microbial and plant, origin. These crystal structures, coupled with in vitro assays, provide a, basis for understanding and potentially manipulating the regio-specific, prenylation of aromatic small molecules using this structurally unique, family of aromatic PTases.

The anti-oxidant naphterpin is a natural product containing a polyketide-based aromatic core with an attached 10-carbon geranyl group derived from isoprenoid (terpene) metabolism. Hybrid natural products such as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl) or 15-carbon (farnesyl) isoprenoid chains possess biological activities distinct from their non-prenylated aromatic precursors. These hybrid natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of aromatic prenyltransferases (PTases) responsible for prenyl group attachment have only recently been isolated and characterized. Here we report the gene identification, biochemical characterization and high-resolution X-ray crystal structures of an architecturally novel aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and carbon-oxygen-based prenylation of a diverse collection of hydroxyl-containing aromatic acceptors of synthetic, microbial and plant origin. These crystal structures, coupled with in vitro assays, provide a basis for understanding and potentially manipulating the regio-specific prenylation of aromatic small molecules using this structurally unique family of aromatic PTases.

==About this Structure==

1ZB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with MG, NO3, GST and DIN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1ZB6 OCA].

1ZB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=GST:'>GST</scene> and <scene name='pdbligand=DIN:'>DIN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB6 OCA].

==Reference==

Line 14:

[[Category: Streptomyces sp.]]

[[Category: Kuzuyama, T.]]

[[Category: Noel, J.P.]]

[[Category: Noel, J P.]]

[[Category: Richard, S.B.]]

[[Category: Richard, S B.]]

[[Category: DIN]]

[[Category: GST]]

Line 23:

[[Category: pt-barrel]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 04~~:48~~:21 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:48 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1zb6.gif|left|200px]]<br /><applet load="1zb6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1zb6.gif|left|200px]]<br /><applet load="1zb6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1zb6, resolution 1.95&Aring;" />
 '''Co-Crystal Structure of ORF2 an Aromatic Prenyl Transferase from Streptomyces sp. strain cl190 complexed with GSPP and 1,6-dihydroxynaphtalene'''<br />
 ==Overview==
-The anti-oxidant naphterpin is a natural product containing a, polyketide-based aromatic core with an attached 10-carbon geranyl group, derived from isoprenoid (terpene) metabolism. Hybrid natural products such, as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl), or 15-carbon (farnesyl) isoprenoid chains possess biological activities, distinct from their non-prenylated aromatic precursors. These hybrid, natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of, aromatic prenyltransferases (PTases) responsible for prenyl group, attachment have only recently been isolated and characterized. Here we, report the gene identification, biochemical characterization and, high-resolution X-ray crystal structures of an architecturally novel, aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates, and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a, 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin, biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and, carbon-oxygen-based prenylation of a diverse collection of, hydroxyl-containing aromatic acceptors of synthetic, microbial and plant, origin. These crystal structures, coupled with in vitro assays, provide a, basis for understanding and potentially manipulating the regio-specific, prenylation of aromatic small molecules using this structurally unique, family of aromatic PTases.
+The anti-oxidant naphterpin is a natural product containing a polyketide-based aromatic core with an attached 10-carbon geranyl group derived from isoprenoid (terpene) metabolism. Hybrid natural products such as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl) or 15-carbon (farnesyl) isoprenoid chains possess biological activities distinct from their non-prenylated aromatic precursors. These hybrid natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of aromatic prenyltransferases (PTases) responsible for prenyl group attachment have only recently been isolated and characterized. Here we report the gene identification, biochemical characterization and high-resolution X-ray crystal structures of an architecturally novel aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and carbon-oxygen-based prenylation of a diverse collection of hydroxyl-containing aromatic acceptors of synthetic, microbial and plant origin. These crystal structures, coupled with in vitro assays, provide a basis for understanding and potentially manipulating the regio-specific prenylation of aromatic small molecules using this structurally unique family of aromatic PTases.
 ==About this Structure==
-ZB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with MG, NO3, GST and DIN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZB6 OCA].
+ZB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=GST:'>GST</scene> and <scene name='pdbligand=DIN:'>DIN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZB6 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Streptomyces sp.]]
 [[Category: Kuzuyama, T.]]
-[[Category: Noel, J.P.]]
+[[Category: Noel, J P.]]
-[[Category: Richard, S.B.]]
+[[Category: Richard, S B.]]
 [[Category: DIN]]
 [[Category: GST]]
@@ Line 23: / Line 23: @@
 [[Category: pt-barrel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:48:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:48 2008''