2a4n: Difference between revisions

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==Overview==

The aminoglycoside-modifying enzyme aminoglycoside 6'-N-acetyltransferase, type Ii [AAC(6')-Ii] has been crystallized with its cofactor coenzyme A in, space group C222(1), with unit-cell parameters a = 71.5, b = 127.4, c =, 76.9 A and one physiologically relevant dimer species per asymmetric unit., The space group previously observed for this complex was P2(1)2(1)2(1), with two dimers per asymmetric unit. By comparing the six available, protomer structures of the AAC(6')-Ii-CoA complex, it has been possible to, identify regions of plasticity within the protein. Normal-mode analysis of, this complex suggests that this plasticity is not an artefact of, crystal-packing forces, but that the region of the protomer that displays, multiple conformations is intrinsically flexible. It is conjectured that, the flexibility is relevant for the cooperative activity observed for the, enzyme.

The aminoglycoside-modifying enzyme aminoglycoside 6'-N-acetyltransferase type Ii [AAC(6')-Ii] has been crystallized with its cofactor coenzyme A in space group C222(1), with unit-cell parameters a = 71.5, b = 127.4, c = 76.9 A and one physiologically relevant dimer species per asymmetric unit. The space group previously observed for this complex was P2(1)2(1)2(1), with two dimers per asymmetric unit. By comparing the six available protomer structures of the AAC(6')-Ii-CoA complex, it has been possible to identify regions of plasticity within the protein. Normal-mode analysis of this complex suggests that this plasticity is not an artefact of crystal-packing forces, but that the region of the protomer that displays multiple conformations is intrinsically flexible. It is conjectured that the flexibility is relevant for the cooperative activity observed for the enzyme.

==About this Structure==

Line 14:

[[Category: Enterococcus faecium]]

[[Category: Single protein]]

[[Category: Berghuis, A.M.]]

[[Category: Berghuis, A M.]]

[[Category: Breitbach, C.]]

[[Category: Burk, D.L.]]

[[Category: Burk, D L.]]

[[Category: Xiong, B.]]

[[Category: COA]]

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[[Category: n-acetyl transferase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:36 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The aminoglycoside-modifying enzyme aminoglycoside 6'-N-acetyltransferase, type Ii [AAC(6')-Ii] has been crystallized with its cofactor coenzyme A in, space group C222(1), with unit-cell parameters a = 71.5, b = 127.4, c =, 76.9 A and one physiologically relevant dimer species per asymmetric unit., The space group previously observed for this complex was P2(1)2(1)2(1), with two dimers per asymmetric unit. By comparing the six available, protomer structures of the AAC(6')-Ii-CoA complex, it has been possible to, identify regions of plasticity within the protein. Normal-mode analysis of, this complex suggests that this plasticity is not an artefact of, crystal-packing forces, but that the region of the protomer that displays, multiple conformations is intrinsically flexible. It is conjectured that, the flexibility is relevant for the cooperative activity observed for the, enzyme.
+The aminoglycoside-modifying enzyme aminoglycoside 6'-N-acetyltransferase type Ii [AAC(6')-Ii] has been crystallized with its cofactor coenzyme A in space group C222(1), with unit-cell parameters a = 71.5, b = 127.4, c = 76.9 A and one physiologically relevant dimer species per asymmetric unit. The space group previously observed for this complex was P2(1)2(1)2(1), with two dimers per asymmetric unit. By comparing the six available protomer structures of the AAC(6')-Ii-CoA complex, it has been possible to identify regions of plasticity within the protein. Normal-mode analysis of this complex suggests that this plasticity is not an artefact of crystal-packing forces, but that the region of the protomer that displays multiple conformations is intrinsically flexible. It is conjectured that the flexibility is relevant for the cooperative activity observed for the enzyme.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Enterococcus faecium]]
 [[Category: Single protein]]
-[[Category: Berghuis, A.M.]]
+[[Category: Berghuis, A M.]]
 [[Category: Breitbach, C.]]
-[[Category: Burk, D.L.]]
+[[Category: Burk, D L.]]
 [[Category: Xiong, B.]]
 [[Category: COA]]
@@ Line 23: / Line 23: @@
 [[Category: n-acetyl transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:54:20 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:36 2008''