2aql: Difference between revisions

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[[Image:2aql.gif|left|200px]]<br />

[[Image:2aql.gif|left|200px]]<br /><applet load="2aql" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2aql" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2aql, resolution 2.30Å" />

'''Crystal Structure of the MRG15 MRG domain'''<br />

==Overview==

The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and, tumor suppression at the cellular level. These proteins are defined by the, approximately 20 kDa MRG domain that binds a plethora of transcriptional, regulators and chromatin-remodeling factors, including the histone, deacetylase transcriptional corepressor mSin3A and the novel nuclear, protein PAM14, and they are also known components of the Tip60/NuA4, complex via interactions with the MRG binding protein (MRGBP). We present, here the crystal structure of a prototypic MRG domain from human MRG15, whose core consists of two orthogonal helix hairpins. Despite the lack of, sequence similarity, the core structure has surprisingly striking homology, to a DNA-interacting domain of the tyrosine site-specific recombinases, XerD, lambda integrase, and Cre. Site-directed mutagenesis studies based, on the X-ray structure and bioinformatics identified key residues involved, in the binding of PAM14 and MRGBP.

The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and tumor suppression at the cellular level. These proteins are defined by the approximately 20 kDa MRG domain that binds a plethora of transcriptional regulators and chromatin-remodeling factors, including the histone deacetylase transcriptional corepressor mSin3A and the novel nuclear protein PAM14, and they are also known components of the Tip60/NuA4 complex via interactions with the MRG binding protein (MRGBP). We present here the crystal structure of a prototypic MRG domain from human MRG15 whose core consists of two orthogonal helix hairpins. Despite the lack of sequence similarity, the core structure has surprisingly striking homology to a DNA-interacting domain of the tyrosine site-specific recombinases XerD, lambda integrase, and Cre. Site-directed mutagenesis studies based on the X-ray structure and bioinformatics identified key residues involved in the binding of PAM14 and MRGBP.

==About this Structure==

2AQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2AQL OCA].

2AQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AQL OCA].

==Reference==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Bowman, B.R.]]

[[Category: Bowman, B R.]]

[[Category: Quiocho, F.A.]]

[[Category: Quiocho, F A.]]

[[Category: chromatin remodeling]]

[[Category: core binding domain]]

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[[Category: recombinase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 20~~:53:~~39 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:29:52 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2aql.gif|left|200px]]<br />
+[[Image:2aql.gif|left|200px]]<br /><applet load="2aql" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2aql" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2aql, resolution 2.30&Aring;" />
 '''Crystal Structure of the MRG15 MRG domain'''<br />
 ==Overview==
-The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and, tumor suppression at the cellular level. These proteins are defined by the, approximately 20 kDa MRG domain that binds a plethora of transcriptional, regulators and chromatin-remodeling factors, including the histone, deacetylase transcriptional corepressor mSin3A and the novel nuclear, protein PAM14, and they are also known components of the Tip60/NuA4, complex via interactions with the MRG binding protein (MRGBP). We present, here the crystal structure of a prototypic MRG domain from human MRG15, whose core consists of two orthogonal helix hairpins. Despite the lack of, sequence similarity, the core structure has surprisingly striking homology, to a DNA-interacting domain of the tyrosine site-specific recombinases, XerD, lambda integrase, and Cre. Site-directed mutagenesis studies based, on the X-ray structure and bioinformatics identified key residues involved, in the binding of PAM14 and MRGBP.
+The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and tumor suppression at the cellular level. These proteins are defined by the approximately 20 kDa MRG domain that binds a plethora of transcriptional regulators and chromatin-remodeling factors, including the histone deacetylase transcriptional corepressor mSin3A and the novel nuclear protein PAM14, and they are also known components of the Tip60/NuA4 complex via interactions with the MRG binding protein (MRGBP). We present here the crystal structure of a prototypic MRG domain from human MRG15 whose core consists of two orthogonal helix hairpins. Despite the lack of sequence similarity, the core structure has surprisingly striking homology to a DNA-interacting domain of the tyrosine site-specific recombinases XerD, lambda integrase, and Cre. Site-directed mutagenesis studies based on the X-ray structure and bioinformatics identified key residues involved in the binding of PAM14 and MRGBP.
 ==About this Structure==
-AQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AQL OCA].
+AQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AQL OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Bowman, B.R.]]
+[[Category: Bowman, B R.]]
-[[Category: Quiocho, F.A.]]
+[[Category: Quiocho, F A.]]
 [[Category: chromatin remodeling]]
 [[Category: core binding domain]]
@@ Line 23: / Line 22: @@
 [[Category: recombinase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:53:39 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:29:52 2008''