2b3j: Difference between revisions

Line 4:

==Overview==

Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic, deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different, arginine codons in mRNA. In addition, inosine is also introduced at the, wobble position of multiple eukaryotic tRNAs. The genes encoding these, deaminases are essential in bacteria and yeast, demonstrating the, importance of their biological activity. Here we report the, crystallization and structure determination to 2.0 A of Staphylococcus, aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing, nebularine, a non-hydrolyzable adenosine analog, at the wobble position., The cocrystal structure reveals the basis for both sequence and structure, specificity in the interactions of TadA with RNA, and it additionally, provides insight into the active site architecture that promotes efficient, hydrolytic deamination.

Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryotic tRNAs. The genes encoding these deaminases are essential in bacteria and yeast, demonstrating the importance of their biological activity. Here we report the crystallization and structure determination to 2.0 A of Staphylococcus aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing nebularine, a non-hydrolyzable adenosine analog, at the wobble position. The cocrystal structure reveals the basis for both sequence and structure specificity in the interactions of TadA with RNA, and it additionally provides insight into the active site architecture that promotes efficient hydrolytic deamination.

==About this Structure==

Line 13:

[[Category: Single protein]]

[[Category: Staphylococcus aureus subsp. aureus mu50]]

[[Category: Losey, H.C.]]

[[Category: Losey, H C.]]

[[Category: Ruthenburg, A.J.]]

[[Category: Ruthenburg, A J.]]

[[Category: Verdine, G.L.]]

[[Category: Verdine, G L.]]

[[Category: GOL]]

[[Category: ZN]]

Line 22:

[[Category: rna stem-loop]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jan 29 18~~:15:40 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:46 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic, deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different, arginine codons in mRNA. In addition, inosine is also introduced at the, wobble position of multiple eukaryotic tRNAs. The genes encoding these, deaminases are essential in bacteria and yeast, demonstrating the, importance of their biological activity. Here we report the, crystallization and structure determination to 2.0 A of Staphylococcus, aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing, nebularine, a non-hydrolyzable adenosine analog, at the wobble position., The cocrystal structure reveals the basis for both sequence and structure, specificity in the interactions of TadA with RNA, and it additionally, provides insight into the active site architecture that promotes efficient, hydrolytic deamination.
+Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryotic tRNAs. The genes encoding these deaminases are essential in bacteria and yeast, demonstrating the importance of their biological activity. Here we report the crystallization and structure determination to 2.0 A of Staphylococcus aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing nebularine, a non-hydrolyzable adenosine analog, at the wobble position. The cocrystal structure reveals the basis for both sequence and structure specificity in the interactions of TadA with RNA, and it additionally provides insight into the active site architecture that promotes efficient hydrolytic deamination.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Staphylococcus aureus subsp. aureus mu50]]
-[[Category: Losey, H.C.]]
+[[Category: Losey, H C.]]
-[[Category: Ruthenburg, A.J.]]
+[[Category: Ruthenburg, A J.]]
-[[Category: Verdine, G.L.]]
+[[Category: Verdine, G L.]]
 [[Category: GOL]]
 [[Category: ZN]]
@@ Line 22: / Line 22: @@
 [[Category: rna stem-loop]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:15:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:46 2008''