2bc3: Difference between revisions

Line 1:

[[Image:2bc3.gif|left|200px]]<br /><applet load="2bc3" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2bc3.gif|left|200px]]<br /><applet load="2bc3" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2bc3, resolution 1.54Å" />

'''T7-tagged full-length streptavidin'''<br />

==Overview==

The structure of a full-length streptavidin has been determined at 1.7 A, resolution and shows that the 20 residue extension at the C terminus forms, a well-ordered polypeptide loop on the surface of the tetramer. Residues, 150-153 of the extension are bound to the ligand-binding site, possibly, competing with exogenous ligands. The binding mode of these residues is, compared with that of biotin and peptidic ligands. The observed structure, helps to rationalize the observations that full-length mature streptavidin, binds biotinylated macromolecules with reduced affinity.

The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.

==About this Structure==

2BC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].

2BC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].

==Reference==

Line 14:

[[Category: Streptomyces avidinii]]

[[Category: Humbert, N.]]

[[Category: Stenkamp, R.E.]]

[[Category: Stenkamp, R E.]]

[[Category: Trong, I.Le.]]

[[Category: Trong, I Le.]]

[[Category: Ward, T.R.]]

[[Category: Ward, T R.]]

[[Category: GOL]]

[[Category: SO4]]

Line 22:

[[Category: t7 tag]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 08:42:~~49 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:18 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2bc3.gif|left|200px]]<br /><applet load="2bc3" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bc3.gif|left|200px]]<br /><applet load="2bc3" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2bc3, resolution 1.54&Aring;" />
 '''T7-tagged full-length streptavidin'''<br />
 ==Overview==
-The structure of a full-length streptavidin has been determined at 1.7 A, resolution and shows that the 20 residue extension at the C terminus forms, a well-ordered polypeptide loop on the surface of the tetramer. Residues, 150-153 of the extension are bound to the ligand-binding site, possibly, competing with exogenous ligands. The binding mode of these residues is, compared with that of biotin and peptidic ligands. The observed structure, helps to rationalize the observations that full-length mature streptavidin, binds biotinylated macromolecules with reduced affinity.
+The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.
 ==About this Structure==
-BC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].
+BC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Streptomyces avidinii]]
 [[Category: Humbert, N.]]
-[[Category: Stenkamp, R.E.]]
+[[Category: Stenkamp, R E.]]
-[[Category: Trong, I.Le.]]
+[[Category: Trong, I Le.]]
-[[Category: Ward, T.R.]]
+[[Category: Ward, T R.]]
 [[Category: GOL]]
 [[Category: SO4]]
@@ Line 22: / Line 22: @@
 [[Category: t7 tag]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:42:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:18 2008''