2bhn: Difference between revisions

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==Overview==

The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA, (dsDNA) within asymmetric branched DNA substrates and play an essential, role in nucleotide excision repair, recombination and genome integrity. We, report the structure of an archaeal XPF homodimer alone and bound to, dsDNA. Superposition of these structures reveals a large domain movement, upon binding DNA, indicating how the (HhH)(2) domain and the nuclease, domain are coupled to allow the recognition of, double-stranded/single-stranded DNA junctions. We identify two, nonequivalent DNA-binding sites and propose a model in which XPF distorts, the 3' flap substrate in order to engage both binding sites and promote, strand cleavage. The model rationalises published biochemical data and, implies a novel role for the ERCC1 subunit of eukaryotic XPF complexes.

The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA (dsDNA) within asymmetric branched DNA substrates and play an essential role in nucleotide excision repair, recombination and genome integrity. We report the structure of an archaeal XPF homodimer alone and bound to dsDNA. Superposition of these structures reveals a large domain movement upon binding DNA, indicating how the (HhH)(2) domain and the nuclease domain are coupled to allow the recognition of double-stranded/single-stranded DNA junctions. We identify two nonequivalent DNA-binding sites and propose a model in which XPF distorts the 3' flap substrate in order to engage both binding sites and promote strand cleavage. The model rationalises published biochemical data and implies a novel role for the ERCC1 subunit of eukaryotic XPF complexes.

==About this Structure==

Line 14:

[[Category: Single protein]]

[[Category: Fadden, A.]]

[[Category: Knowles, P.P.]]

[[Category: Knowles, P P.]]

[[Category: Lally, J.]]

[[Category: Mcdonald, N.Q.]]

[[Category: Mcdonald, N Q.]]

[[Category: Murray-Rust, J.]]

[[Category: Newman, M.]]

[[Category: Rudolf, J.]]

[[Category: White, M.F.]]

[[Category: White, M F.]]

[[Category: hydrolase]]

[[Category: nucleotide excision repair]]

[[Category: structure specific endonuclease]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA, (dsDNA) within asymmetric branched DNA substrates and play an essential, role in nucleotide excision repair, recombination and genome integrity. We, report the structure of an archaeal XPF homodimer alone and bound to, dsDNA. Superposition of these structures reveals a large domain movement, upon binding DNA, indicating how the (HhH)(2) domain and the nuclease, domain are coupled to allow the recognition of, double-stranded/single-stranded DNA junctions. We identify two, nonequivalent DNA-binding sites and propose a model in which XPF distorts, the 3' flap substrate in order to engage both binding sites and promote, strand cleavage. The model rationalises published biochemical data and, implies a novel role for the ERCC1 subunit of eukaryotic XPF complexes.
+The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA (dsDNA) within asymmetric branched DNA substrates and play an essential role in nucleotide excision repair, recombination and genome integrity. We report the structure of an archaeal XPF homodimer alone and bound to dsDNA. Superposition of these structures reveals a large domain movement upon binding DNA, indicating how the (HhH)(2) domain and the nuclease domain are coupled to allow the recognition of double-stranded/single-stranded DNA junctions. We identify two nonequivalent DNA-binding sites and propose a model in which XPF distorts the 3' flap substrate in order to engage both binding sites and promote strand cleavage. The model rationalises published biochemical data and implies a novel role for the ERCC1 subunit of eukaryotic XPF complexes.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Fadden, A.]]
-[[Category: Knowles, P.P.]]
+[[Category: Knowles, P P.]]
 [[Category: Lally, J.]]
-[[Category: Mcdonald, N.Q.]]
+[[Category: Mcdonald, N Q.]]
 [[Category: Murray-Rust, J.]]
 [[Category: Newman, M.]]
 [[Category: Rudolf, J.]]
-[[Category: White, M.F.]]
+[[Category: White, M F.]]
 [[Category: hydrolase]]
 [[Category: nucleotide excision repair]]
 [[Category: structure specific endonuclease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:24:30 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:54 2008''