2biu: Difference between revisions

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==Overview==

In the pharmaceutical industry, knowledge of the three-dimensional, structure of a specific target facilitates the drug-discovery process., Despite possessing favoured analytical properties such as high purity and, monodispersion in light scattering, some proteins are not capable of, forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of, cyclophilin that is expressed in the mitochondria, was selected as a drug, target for the treatment of cardiac disorders. As the wild-type enzyme, defied all attempts at crystallization, protein engineering on the enzyme, surface was performed. The K133I mutant gave crystals that diffracted to, 1.7 A resolution using in-house X-ray facilities and were suitable for, soaking experiments. The crystals were very robust and diffraction was, maintained after soaking in 25% DMSO solution: excellent conditions for, the rapid analysis of complex structures including crystallographic, fragment screening.

In the pharmaceutical industry, knowledge of the three-dimensional structure of a specific target facilitates the drug-discovery process. Despite possessing favoured analytical properties such as high purity and monodispersion in light scattering, some proteins are not capable of forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of cyclophilin that is expressed in the mitochondria, was selected as a drug target for the treatment of cardiac disorders. As the wild-type enzyme defied all attempts at crystallization, protein engineering on the enzyme surface was performed. The K133I mutant gave crystals that diffracted to 1.7 A resolution using in-house X-ray facilities and were suitable for soaking experiments. The crystals were very robust and diffraction was maintained after soaking in 25% DMSO solution: excellent conditions for the rapid analysis of complex structures including crystallographic fragment screening.

==Disease==

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[[Category: mitochondrial protein]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:16 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-In the pharmaceutical industry, knowledge of the three-dimensional, structure of a specific target facilitates the drug-discovery process., Despite possessing favoured analytical properties such as high purity and, monodispersion in light scattering, some proteins are not capable of, forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of, cyclophilin that is expressed in the mitochondria, was selected as a drug, target for the treatment of cardiac disorders. As the wild-type enzyme, defied all attempts at crystallization, protein engineering on the enzyme, surface was performed. The K133I mutant gave crystals that diffracted to, 1.7 A resolution using in-house X-ray facilities and were suitable for, soaking experiments. The crystals were very robust and diffraction was, maintained after soaking in 25% DMSO solution: excellent conditions for, the rapid analysis of complex structures including crystallographic, fragment screening.
+In the pharmaceutical industry, knowledge of the three-dimensional structure of a specific target facilitates the drug-discovery process. Despite possessing favoured analytical properties such as high purity and monodispersion in light scattering, some proteins are not capable of forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of cyclophilin that is expressed in the mitochondria, was selected as a drug target for the treatment of cardiac disorders. As the wild-type enzyme defied all attempts at crystallization, protein engineering on the enzyme surface was performed. The K133I mutant gave crystals that diffracted to 1.7 A resolution using in-house X-ray facilities and were suitable for soaking experiments. The crystals were very robust and diffraction was maintained after soaking in 25% DMSO solution: excellent conditions for the rapid analysis of complex structures including crystallographic fragment screening.
 ==Disease==
@@ Line 28: / Line 28: @@
 [[Category: mitochondrial protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:24:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:16 2008''