2bkj: Difference between revisions

[[Image:2bkj.jpg|left|200px]]<br /><applet load="2bkj" size="350" color="white" frame="true" align="right" spinBox="true"  

The 2.1 A resolution crystal structure of flavin reductase P with the inhibitor nicotinamide adenine dinucleotide (NAD) bound in the active site has been determined. NAD adopts a novel, folded conformation in which the nicotinamide and adenine rings stack in parallel with an inter-ring distance of 3.6 A. The pyrophosphate binds next to the flavin cofactor isoalloxazine, while the stacked nicotinamide/adenine moiety faces away from the flavin. The observed NAD conformation is quite different from the extended conformations observed in other enzyme/NAD(P) structures; however, it resembles the conformation proposed for NAD in solution. The flavin reductase P/NAD structure provides new information about the conformational diversity of NAD, which is important for understanding catalysis. This structure offers the first crystallographic evidence of a folded NAD with ring stacking, and it is the first enzyme structure containing an FMN cofactor interacting with NAD(P). Analysis of the structure suggests a possible dynamic mechanism underlying NADPH substrate specificity and product release that involves unfolding and folding of NADP(H).

2BKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKJ OCA].  

[[Category: Krause, K L.]]

[[Category: TU, S C.]]

[[Category: Tanner, J J.]]

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