2bps: Difference between revisions

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==Overview==

The YukD protein in Bacillus subtilis was identified in a hidden Markov, model (HMM) search as being related in sequence to ubiquitin. By solving, the crystal structure we show that YukD adopts a fold that is most closely, related to ubiquitin, yet has the shortest C-terminal tail of all known, ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was, disrupted without an obvious phenotypic effect and an inducible copy, encoding a C-Myc and His-tagged version of the protein was introduced at, the ectopic locus amyE. Conjugation assays performed both in vitro and in, vivo indicate that YukD lacks the capacity for covalent bond formation, with other proteins.

The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins.

==About this Structure==

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[[Category: Bacillus subtilis]]

[[Category: Single protein]]

[[Category: Ent, F.Van.Den.]]

[[Category: Ent, F Van Den.]]

[[Category: Lowe, J.]]

[[Category: ubiquitin]]

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[[Category: yukd]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The YukD protein in Bacillus subtilis was identified in a hidden Markov, model (HMM) search as being related in sequence to ubiquitin. By solving, the crystal structure we show that YukD adopts a fold that is most closely, related to ubiquitin, yet has the shortest C-terminal tail of all known, ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was, disrupted without an obvious phenotypic effect and an inducible copy, encoding a C-Myc and His-tagged version of the protein was introduced at, the ectopic locus amyE. Conjugation assays performed both in vitro and in, vivo indicate that YukD lacks the capacity for covalent bond formation, with other proteins.
+The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Bacillus subtilis]]
 [[Category: Single protein]]
-[[Category: Ent, F.Van.Den.]]
+[[Category: Ent, F Van Den.]]
 [[Category: Lowe, J.]]
 [[Category: ubiquitin]]
@@ Line 19: / Line 19: @@
 [[Category: yukd]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:28:36 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:40:17 2008''