2bv8: Difference between revisions

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==Overview==

Phycocyanin is a phycobiliprotein involved in light harvesting and, conduction of light to the reaction centers in cyanobacteria and red, algae. The structure of C-phycocyanin from Gracilaria chilensis was solved, by X-ray crystallography at 2.0 A resolution in space group P2(1). An, interaction model between two PC heterohexamers was built, followed by, molecular dynamic refinement. The best model showed an inter-hexamer, rotation of 23 degrees . The coordinates of a PC heterohexamer, (alphabeta)(6) and of the PC-PC complex were used to perform energy, transfer calculations between chromophores pairs using the fluorescence, resonance energy transfer approach (FRET). Two main intra PC, ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and, (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC, ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)), pathways were proposed based on the values of the energy transfer, constants calculated for all the chromophore pairs in the hexamer and in, the complex.

Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.

==About this Structure==

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[[Category: phycocyanobilin]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Phycocyanin is a phycobiliprotein involved in light harvesting and, conduction of light to the reaction centers in cyanobacteria and red, algae. The structure of C-phycocyanin from Gracilaria chilensis was solved, by X-ray crystallography at 2.0 A resolution in space group P2(1). An, interaction model between two PC heterohexamers was built, followed by, molecular dynamic refinement. The best model showed an inter-hexamer, rotation of 23 degrees . The coordinates of a PC heterohexamer, (alphabeta)(6) and of the PC-PC complex were used to perform energy, transfer calculations between chromophores pairs using the fluorescence, resonance energy transfer approach (FRET). Two main intra PC, ((I)beta(3)(82)--&gt;(I)alpha(1)(84)--&gt;(I)alpha(5)(84)--&gt;(I)beta(6)(82) and, (I)beta(3)(153)--&gt;(I)beta(5)(153)) and two main inter PC, ((I)beta(6)(82)--&gt;(II)beta(3)(82) and (I)beta(5)(153)--&gt;(II)beta(3)(153)), pathways were proposed based on the values of the energy transfer, constants calculated for all the chromophore pairs in the hexamer and in, the complex.
+Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)--&gt;(I)alpha(1)(84)--&gt;(I)alpha(5)(84)--&gt;(I)beta(6)(82) and (I)beta(3)(153)--&gt;(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)--&gt;(II)beta(3)(82) and (I)beta(5)(153)--&gt;(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.
 ==About this Structure==
@@ Line 28: / Line 28: @@
 [[Category: phycocyanobilin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:31:18 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:56 2008''