2c0l: Difference between revisions

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==Overview==

Peroxisomes require the translocation of folded and functional target, proteins of various sizes across the peroxisomal membrane. We have, investigated the structure and function of the principal import receptor, Pex5p, which recognizes targets bearing a C-terminal peroxisomal targeting, signal type 1. Crystal structures of the receptor in the presence and, absence of a peroxisomal target, sterol carrier protein 2, reveal major, structural changes from an open, snail-like conformation into a closed, circular conformation. These changes are caused by a long loop C terminal, to the 7-fold tetratricopeptide repeat segments. Mutations in residues of, this loop lead to defects in peroxisomal import in human fibroblasts. The, structure of the receptor/cargo complex demonstrates that the primary, receptor-binding site of the cargo is structurally and topologically, autonomous, enabling the cargo to retain its native structure and, function.

Peroxisomes require the translocation of folded and functional target proteins of various sizes across the peroxisomal membrane. We have investigated the structure and function of the principal import receptor Pex5p, which recognizes targets bearing a C-terminal peroxisomal targeting signal type 1. Crystal structures of the receptor in the presence and absence of a peroxisomal target, sterol carrier protein 2, reveal major structural changes from an open, snail-like conformation into a closed, circular conformation. These changes are caused by a long loop C terminal to the 7-fold tetratricopeptide repeat segments. Mutations in residues of this loop lead to defects in peroxisomal import in human fibroblasts. The structure of the receptor/cargo complex demonstrates that the primary receptor-binding site of the cargo is structurally and topologically autonomous, enabling the cargo to retain its native structure and function.

==Disease==

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[[Category: Protein complex]]

[[Category: Kursula, P.]]

[[Category: Stanley, W.A.]]

[[Category: Stanley, W A.]]

[[Category: Wilmanns, M.]]

[[Category: alternative initiation]]

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[[Category: zellweger syndrome]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:43:36 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Peroxisomes require the translocation of folded and functional target, proteins of various sizes across the peroxisomal membrane. We have, investigated the structure and function of the principal import receptor, Pex5p, which recognizes targets bearing a C-terminal peroxisomal targeting, signal type 1. Crystal structures of the receptor in the presence and, absence of a peroxisomal target, sterol carrier protein 2, reveal major, structural changes from an open, snail-like conformation into a closed, circular conformation. These changes are caused by a long loop C terminal, to the 7-fold tetratricopeptide repeat segments. Mutations in residues of, this loop lead to defects in peroxisomal import in human fibroblasts. The, structure of the receptor/cargo complex demonstrates that the primary, receptor-binding site of the cargo is structurally and topologically, autonomous, enabling the cargo to retain its native structure and, function.
+Peroxisomes require the translocation of folded and functional target proteins of various sizes across the peroxisomal membrane. We have investigated the structure and function of the principal import receptor Pex5p, which recognizes targets bearing a C-terminal peroxisomal targeting signal type 1. Crystal structures of the receptor in the presence and absence of a peroxisomal target, sterol carrier protein 2, reveal major structural changes from an open, snail-like conformation into a closed, circular conformation. These changes are caused by a long loop C terminal to the 7-fold tetratricopeptide repeat segments. Mutations in residues of this loop lead to defects in peroxisomal import in human fibroblasts. The structure of the receptor/cargo complex demonstrates that the primary receptor-binding site of the cargo is structurally and topologically autonomous, enabling the cargo to retain its native structure and function.
 ==Disease==
@@ Line 17: / Line 17: @@
 [[Category: Protein complex]]
 [[Category: Kursula, P.]]
-[[Category: Stanley, W.A.]]
+[[Category: Stanley, W A.]]
 [[Category: Wilmanns, M.]]
 [[Category: alternative initiation]]
@@ Line 33: / Line 33: @@
 [[Category: zellweger syndrome]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:17:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:43:36 2008''