2c4b: Difference between revisions
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==Overview== | ==Overview== | ||
We present a fusion system suited to determine the crystal structure of | We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Ribonuclease T(1)]] | [[Category: Ribonuclease T(1)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Heinz, D | [[Category: Heinz, D W.]] | ||
[[Category: Kolmar, H.]] | [[Category: Kolmar, H.]] | ||
[[Category: Niemann, H | [[Category: Niemann, H H.]] | ||
[[Category: Schmoldt, H | [[Category: Schmoldt, H U.]] | ||
[[Category: Wentzel, A.]] | [[Category: Wentzel, A.]] | ||
[[Category: 2PE]] | [[Category: 2PE]] | ||
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[[Category: squash inhibitor]] | [[Category: squash inhibitor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:44:43 2008'' | ||
