2chj: Difference between revisions

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==Overview==

We have determined the NMR solution structures of the quadruplexes formed, by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid), modified G-residues. Both structures are tetrameric, parallel and, right-handed and the native global fold of the corresponding DNA, quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars., In particular, a distinct change in the sugar-phosphate backbone is, observed at the G2pL3 and L2pL3 base steps and sequence dependent changes, in the twist between tetrads are also seen. Both the LNA modified, quadruplexes have raised thermostability as compared to the DNA, quadruplex. The quadruplex-forming capability of d(TGLGLT) is of, particular interest as it expands the design flexibility for stable, parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with, DNA or other modified nucleic acids. As such, LNA-based quadruplexes can, be decorated by a variety of chemical modifications. Such LNA quadruplex, scaffolds might find applications in the developing field of, nanobiotechnology.

We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology.

==About this Structure==

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NMR solution structures of LNA (locked nucleic acid) modified quadruplexes., Nielsen JT, Arar K, Petersen M, Nucleic Acids Res. 2006 Apr 13;34(7):2006-14. Print 2006. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16614450 16614450]

[[Category: Single protein]]

[[Category: Nielsen, J.T.]]

[[Category: Nielsen, J T.]]

[[Category: Petersen, M.]]

[[Category: lna]]

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[[Category: quadruplex]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-We have determined the NMR solution structures of the quadruplexes formed, by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid), modified G-residues. Both structures are tetrameric, parallel and, right-handed and the native global fold of the corresponding DNA, quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars., In particular, a distinct change in the sugar-phosphate backbone is, observed at the G2pL3 and L2pL3 base steps and sequence dependent changes, in the twist between tetrads are also seen. Both the LNA modified, quadruplexes have raised thermostability as compared to the DNA, quadruplex. The quadruplex-forming capability of d(TGLGLT) is of, particular interest as it expands the design flexibility for stable, parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with, DNA or other modified nucleic acids. As such, LNA-based quadruplexes can, be decorated by a variety of chemical modifications. Such LNA quadruplex, scaffolds might find applications in the developing field of, nanobiotechnology.
+We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 NMR solution structures of LNA (locked nucleic acid) modified quadruplexes., Nielsen JT, Arar K, Petersen M, Nucleic Acids Res. 2006 Apr 13;34(7):2006-14. Print 2006. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16614450 16614450]
 [[Category: Single protein]]
-[[Category: Nielsen, J.T.]]
+[[Category: Nielsen, J T.]]
 [[Category: Petersen, M.]]
 [[Category: lna]]
@@ Line 18: / Line 18: @@
 [[Category: quadruplex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:42:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:48:41 2008''