2cn2: Difference between revisions

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==Overview==

The enzymatic degradation of the plant cell wall is central both to the, natural carbon cycle and, increasingly, to environmentally friendly routes, to biomass conversion, including the production of biofuels. The plant, cell wall is a complex composite of cellulose microfibrils embedded in, diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is, one such polysaccharide whose hydrolysis is catalyzed by diverse, xyloglucanases. Here we present the structure of the Clostridium, thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms., The structures, in combination with mutagenesis data on the catalytic, residues and the kinetics and specificity of xyloglucan hydrolysis reveal, a complex subsite specificity accommodating seventeen monosaccharide, moieties of the multibranched substrate in an open substrate binding, terrain.

The enzymatic degradation of the plant cell wall is central both to the natural carbon cycle and, increasingly, to environmentally friendly routes to biomass conversion, including the production of biofuels. The plant cell wall is a complex composite of cellulose microfibrils embedded in diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is one such polysaccharide whose hydrolysis is catalyzed by diverse xyloglucanases. Here we present the structure of the Clostridium thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms. The structures, in combination with mutagenesis data on the catalytic residues and the kinetics and specificity of xyloglucan hydrolysis reveal a complex subsite specificity accommodating seventeen monosaccharide moieties of the multibranched substrate in an open substrate binding terrain.

==About this Structure==

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[[Category: Single protein]]

[[Category: Xyloglucan-specific endo-beta-1,4-glucanase]]

[[Category: Baumann, M.J.]]

[[Category: Baumann, M J.]]

[[Category: Brumer, H.]]

[[Category: Davies, G.J.]]

[[Category: Davies, G J.]]

[[Category: Ferreira, L.M.A.]]

[[Category: Ferreira, L M.A.]]

[[Category: Fontes, C.M.G.A.]]

[[Category: Fontes, C M.G A.]]

[[Category: Guerreiro, C.I.]]

[[Category: Guerreiro, C I.]]

[[Category: Martinez-Fleites, C.]]

[[Category: Prates, J.A.M.]]

[[Category: Prates, J A.M.]]

[[Category: Taylor, E.J.]]

[[Category: Taylor, E J.]]

[[Category: CD]]

[[Category: gh74 family]]

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[[Category: xyloglucanase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:26 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The enzymatic degradation of the plant cell wall is central both to the, natural carbon cycle and, increasingly, to environmentally friendly routes, to biomass conversion, including the production of biofuels. The plant, cell wall is a complex composite of cellulose microfibrils embedded in, diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is, one such polysaccharide whose hydrolysis is catalyzed by diverse, xyloglucanases. Here we present the structure of the Clostridium, thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms., The structures, in combination with mutagenesis data on the catalytic, residues and the kinetics and specificity of xyloglucan hydrolysis reveal, a complex subsite specificity accommodating seventeen monosaccharide, moieties of the multibranched substrate in an open substrate binding, terrain.
+The enzymatic degradation of the plant cell wall is central both to the natural carbon cycle and, increasingly, to environmentally friendly routes to biomass conversion, including the production of biofuels. The plant cell wall is a complex composite of cellulose microfibrils embedded in diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is one such polysaccharide whose hydrolysis is catalyzed by diverse xyloglucanases. Here we present the structure of the Clostridium thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms. The structures, in combination with mutagenesis data on the catalytic residues and the kinetics and specificity of xyloglucan hydrolysis reveal a complex subsite specificity accommodating seventeen monosaccharide moieties of the multibranched substrate in an open substrate binding terrain.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Xyloglucan-specific endo-beta-1,4-glucanase]]
-[[Category: Baumann, M.J.]]
+[[Category: Baumann, M J.]]
 [[Category: Brumer, H.]]
-[[Category: Davies, G.J.]]
+[[Category: Davies, G J.]]
-[[Category: Ferreira, L.M.A.]]
+[[Category: Ferreira, L M.A.]]
-[[Category: Fontes, C.M.G.A.]]
+[[Category: Fontes, C M.G A.]]
-[[Category: Guerreiro, C.I.]]
+[[Category: Guerreiro, C I.]]
 [[Category: Martinez-Fleites, C.]]
-[[Category: Prates, J.A.M.]]
+[[Category: Prates, J A.M.]]
-[[Category: Taylor, E.J.]]
+[[Category: Taylor, E J.]]
 [[Category: CD]]
 [[Category: gh74 family]]
@@ Line 29: / Line 29: @@
 [[Category: xyloglucanase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:37:08 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:26 2008''