Sandbox Reserved 595: Difference between revisions
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ApoE exhibits extensive domain interactions. Hydrogen bonds and salt-bridges act to shield the major LDLR-binding region. This protein's unique topology regulates its tertiary structure in order to solely permit one conformation upon binding in a two-step manner. Lipid-free and partially lipidated ApoE are thwarted from prematurely binding to ApoE receptors by the tertiary structure. Therefore, the optimal receptor-binding affinity of fully lipidated ApoE is guaranteed. An active conformation for biding to members of the low-density lipoprotein receptor family is achieved through binding to lipids and HSPGs (V). | ApoE exhibits extensive domain interactions. Hydrogen bonds and salt-bridges act to shield the major LDLR-binding region. This protein's unique topology regulates its tertiary structure in order to solely permit one conformation upon binding in a two-step manner. Lipid-free and partially lipidated ApoE are thwarted from prematurely binding to ApoE receptors by the tertiary structure. Therefore, the optimal receptor-binding affinity of fully lipidated ApoE is guaranteed. An active conformation for biding to members of the low-density lipoprotein receptor family is achieved through binding to lipids and HSPGs (V). | ||
As was aforementioned, ApoE interacts with multiple partners, including LDLRs, cell-surface HSPGs, ATP-binding cassette protein 1 (ABCA1), and low-density lipoprotein-related proteins (LRPs). It binds with lipids and cholesterol, with high-affinity, to form lipoprotein particles (W). In vivo, ApoE is almost always associated with lipids and cholesterol. Concentrations of lipid-free ApoE are expected to be insignificant (M). | As was aforementioned, ApoE interacts with multiple partners, including LDLRs, cell-surface HSPGs, ATP-binding cassette protein 1 (ABCA1), and low-density lipoprotein-related proteins (LRPs). It binds with lipids and cholesterol, with high-affinity, to form lipoprotein particles (W). ''In vivo'', ApoE is almost always associated with lipids and cholesterol. Concentrations of lipid-free ApoE are expected to be insignificant (M). | ||
==Quarternary Structural Features== | ==Quarternary Structural Features== | ||
