2yol: Difference between revisions

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'''Unreleased structure'''
{{STRUCTURE_2yol|  PDB=2yol  |  SCENE=  }}
===West Nile Virus NS2B-NS3 protease in complex with 3,4- dichlorophenylacetyl-Lys-Lys-GCMA===
{{ABSTRACT_PUBMED_23307694}}


The entry 2yol is ON HOLD until Paper Publication
==Function==
[[http://www.uniprot.org/uniprot/POLG_WNV POLG_WNV]] Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Non-structural protein 1 is involved in virus replication and regulation of the innate immune response (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Non-structural protein 2A may be involved viral RNA replication and capsid assembly (Potential).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Non-structural protein 4A induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the NS3 helicase (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Peptide 2k functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>  Non-structural protein 4B inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref> RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host JAK1 and TYK2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway (By similarity).<ref>PMID:15367621</ref><ref>PMID:15956546</ref><ref>PMID:17267492</ref><ref>PMID:20106931</ref><ref>PMID:19850911</ref>


Authors: Hammamy, M.Z., Haase, C., Hammami, M., Hilgenfeld, R., Steinmetzer, T.
==About this Structure==
[[2yol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/West_nile_virus West nile virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YOL OCA].  


Description: West Nile Virus NS2B-NS3 protease in complex with 3,4-dichlorophenylacetyl-Lys-Lys-GCMA
==Reference==
<references group="xtra"/><references/>
[[Category: West nile virus]]
[[Category: Haase, C.]]
[[Category: Hammami, M.]]
[[Category: Hammamy, M Z.]]
[[Category: Hilgenfeld, R.]]
[[Category: Steinmetzer, T.]]
[[Category: Hydrolase]]
[[Category: Inhibitor]]
[[Category: Serine protease]]
Retrieved from "https://proteopedia.org/2yol"