2dpk: Difference between revisions

Line 1:

[[Image:2dpk.gif|left|200px]]<br /><applet load="2dpk" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2dpk.gif|left|200px]]<br /><applet load="2dpk" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2dpk, resolution 2.50Å" />

'''The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger'''<br />

==Overview==

The Na+/Ca2+ exchanger is a plasma membrane protein that regulates, intracellular Ca2+ levels in cardiac myocytes. Transport activity is, governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large, cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+, to the CBD1 sensory domain results in conformational changes that, stimulate the exchanger to extrude Ca2+. Here, we present a crystal, structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding, site consisting of four Ca2+ ions arranged in a tight planar cluster. This, intricate coordination pattern for a Ca2+ binding cluster is indicative of, a highly sensitive Ca2+ sensor and may represent a general platform for, Ca2+ sensing.

The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing.

==About this Structure==

2DPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with CA, GAI and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2DPK OCA].

2DPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=GAI:'>GAI</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPK OCA].

==Reference==

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[[Category: cis-proline]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 09:42:~~28 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:01:11 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2dpk.gif|left|200px]]<br /><applet load="2dpk" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2dpk.gif|left|200px]]<br /><applet load="2dpk" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2dpk, resolution 2.50&Aring;" />
 '''The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger'''<br />
 ==Overview==
-The Na+/Ca2+ exchanger is a plasma membrane protein that regulates, intracellular Ca2+ levels in cardiac myocytes. Transport activity is, governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large, cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+, to the CBD1 sensory domain results in conformational changes that, stimulate the exchanger to extrude Ca2+. Here, we present a crystal, structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding, site consisting of four Ca2+ ions arranged in a tight planar cluster. This, intricate coordination pattern for a Ca2+ binding cluster is indicative of, a highly sensitive Ca2+ sensor and may represent a general platform for, Ca2+ sensing.
+The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing.
 ==About this Structure==
-DPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with CA, GAI and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DPK OCA].
+DPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=GAI:'>GAI</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPK OCA].
 ==Reference==
@@ Line 23: / Line 23: @@
 [[Category: cis-proline]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:42:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:01:11 2008''