3ki2: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
{{STRUCTURE_3ki2| PDB=3ki2 | SCENE= }} | {{STRUCTURE_3ki2| PDB=3ki2 | SCENE= }} | ||
===Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-G=== | ===Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-G=== | ||
==Function== | |||
[[http://www.uniprot.org/uniprot/Q5EK40_VIBCH Q5EK40_VIBCH]] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref> | |||
==About this Structure== | ==About this Structure== | ||
[[3ki2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KI2 OCA]. | |||
==Reference== | |||
<references group="xtra"/><references/> | |||
[[Category: Vibrio cholerae]] | [[Category: Vibrio cholerae]] | ||
[[Category: Edwards, P R.]] | [[Category: Edwards, P R.]] | ||
| Line 24: | Line 19: | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 02:13, 4 April 2013
Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-G
Function
[Q5EK40_VIBCH] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.[1] [2]
About this Structure
3ki2 is a 1 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA.
Reference
- ↑ Jorgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Rod Merrill A. Cholix toxin, a novel ADP-ribosylating factor from vibrio cholerae. J Biol Chem. 2008 Feb 25;. PMID:18276581 doi:M710008200
- ↑ Turgeon Z, White D, Jorgensen R, Visschedyk D, Fieldhouse RJ, Mangroo D, Merrill AR. Yeast as a tool for characterizing mono-ADP-ribosyltransferase toxins. FEMS Microbiol Lett. 2009 Nov;300(1):97-106. Epub 2009 Aug 31. PMID:19793133 doi:10.1111/j.1574-6968.2009.01777.x
