2erm: Difference between revisions

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==Overview==

The 3D structure of a complex formed by the acidic fibroblast growth, factor (FGF-1) and a specifically designed synthetic heparin, hexasaccharide has been determined by NMR spectroscopy. This, hexasaccharide can substitute natural heparins in FGF-1 mitogenesis, assays, in spite of not inducing any apparent dimerization of the growth, factor. The use of this well defined synthetic heparin analogue has, allowed us to perform a detailed NMR structural analysis of the, heparin-FGF interaction, overcoming the limitations of NMR to deal with, the high molecular mass and heterogeneity of the FGF-1 oligomers formed in, the presence of natural heparin fragments. Our results confirm that, glycosaminoglycans induced FGF-1 dimerization either in a cis or trans, disposition with respect to the heparin chain is not an absolute, requirement for biological activity.

The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin-FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity.

==Disease==

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[[Category: Lozano, R.]]

[[Category: Martin-Lomas, M.]]

[[Category: Nieto, P.M.]]

[[Category: Nieto, P M.]]

[[Category: IPA]]

[[Category: fibroblast growth factor]]

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[[Category: protein-carbohydrate complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Feb 15 17:23:46 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:13:58 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The 3D structure of a complex formed by the acidic fibroblast growth, factor (FGF-1) and a specifically designed synthetic heparin, hexasaccharide has been determined by NMR spectroscopy. This, hexasaccharide can substitute natural heparins in FGF-1 mitogenesis, assays, in spite of not inducing any apparent dimerization of the growth, factor. The use of this well defined synthetic heparin analogue has, allowed us to perform a detailed NMR structural analysis of the, heparin-FGF interaction, overcoming the limitations of NMR to deal with, the high molecular mass and heterogeneity of the FGF-1 oligomers formed in, the presence of natural heparin fragments. Our results confirm that, glycosaminoglycans induced FGF-1 dimerization either in a cis or trans, disposition with respect to the heparin chain is not an absolute, requirement for biological activity.
+The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin-FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity.
 ==Disease==
@@ Line 21: / Line 21: @@
 [[Category: Lozano, R.]]
 [[Category: Martin-Lomas, M.]]
-[[Category: Nieto, P.M.]]
+[[Category: Nieto, P M.]]
 [[Category: IPA]]
 [[Category: fibroblast growth factor]]
@@ Line 27: / Line 27: @@
 [[Category: protein-carbohydrate complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:23:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:13:58 2008''