2pot: Difference between revisions
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{{STRUCTURE_2pot| PDB=2pot | SCENE= }} | {{STRUCTURE_2pot| PDB=2pot | SCENE= }} | ||
===tRNA guanine transglycosylase (TGT) E235Q mutant in complex with guanine=== | ===tRNA guanine transglycosylase (TGT) E235Q mutant in complex with guanine=== | ||
{{ABSTRACT_PUBMED_17949745}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168] | |||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
<ref group="xtra">PMID:017949745</ref><references group="xtra"/> | <ref group="xtra">PMID:017949745</ref><references group="xtra"/><references/> | ||
[[Category: Zymomonas mobilis]] | [[Category: Zymomonas mobilis]] | ||
[[Category: Heine, A.]] | [[Category: Heine, A.]] | ||
[[Category: Klebe, G.]] | [[Category: Klebe, G.]] | ||
Revision as of 10:06, 24 April 2013
tRNA guanine transglycosylase (TGT) E235Q mutant in complex with guanine
Template:ABSTRACT PUBMED 17949745
Function
[TGT_ZYMMO] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
About this Structure
2pot is a 1 chain structure with sequence from Zymomonas mobilis. Full crystallographic information is available from OCA.
Reference
- Tidten N, Stengl B, Heine A, Garcia GA, Klebe G, Reuter K. Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies. J Mol Biol. 2007 Nov 30;374(3):764-76. Epub 2007 Oct 22. PMID:17949745 doi:10.1016/j.jmb.2007.09.062
