2fd4: Difference between revisions
New page: left|200px<br /><applet load="2fd4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fd4, resolution 1.80Å" /> '''Crystal Structure of... |
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[[Image:2fd4.gif|left|200px]]<br /><applet load="2fd4" size=" | [[Image:2fd4.gif|left|200px]]<br /><applet load="2fd4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2fd4, resolution 1.80Å" /> | caption="2fd4, resolution 1.80Å" /> | ||
'''Crystal Structure of AvrPtoB (436-553)'''<br /> | '''Crystal Structure of AvrPtoB (436-553)'''<br /> | ||
==Overview== | ==Overview== | ||
The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The | The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses. | ||
==About this Structure== | ==About this Structure== | ||
2FD4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http:// | 2FD4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FD4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Janjusevic, R.]] | [[Category: Janjusevic, R.]] | ||
[[Category: Stebbins, C | [[Category: Stebbins, C E.]] | ||
[[Category: avrptob]] | [[Category: avrptob]] | ||
[[Category: pseudomonas]] | [[Category: pseudomonas]] | ||
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[[Category: ubiquitin ligase]] | [[Category: ubiquitin ligase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:09 2008'' | ||
Revision as of 15:20, 21 February 2008
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Crystal Structure of AvrPtoB (436-553)
Overview
The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses.
About this Structure
2FD4 is a Single protein structure of sequence from Pseudomonas syringae. Full crystallographic information is available from OCA.
Reference
A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase., Janjusevic R, Abramovitch RB, Martin GB, Stebbins CE, Science. 2006 Jan 13;311(5758):222-6. Epub 2005 Dec 22. PMID:16373536
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