2ff0: Difference between revisions

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[[Image:2ff0.gif|left|200px]]<br /><applet load="2ff0" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2ff0.gif|left|200px]]<br /><applet load="2ff0" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2ff0" />

'''Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter'''<br />

==Overview==

Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear, hormone receptors and is considered a master regulator of reproduction, because it regulates a number of genes encoding reproductive hormones and, enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the, FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all, nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we, describe the solution structure of the SF1 DBD in complex with an atypical, sequence in the proximal promoter region of the inhibin-alpha gene that, encodes a subunit of a reproductive hormone. SF1 forms a specific complex, with the DNA through a bipartite motif binding to the major and minor, grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric, receptors, nerve growth factor-induced-B and estrogen-related receptor 2., However, unlike these receptors, SF1 harbors a helix in the C-terminal, segment of the FTZ-F1 box that interacts with both the core DBD and DNA, and serves as an important determinant of stability of the complex. We, propose that the FTZ-F1 helix along with the core DBD serves as a platform, for interactions with coactivators and other DNA-bound factors in the, vicinity.

Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear hormone receptors and is considered a master regulator of reproduction because it regulates a number of genes encoding reproductive hormones and enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-alpha gene that encodes a subunit of a reproductive hormone. SF1 forms a specific complex with the DNA through a bipartite motif binding to the major and minor grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric receptors, nerve growth factor-induced-B and estrogen-related receptor 2. However, unlike these receptors, SF1 harbors a helix in the C-terminal segment of the FTZ-F1 box that interacts with both the core DBD and DNA and serves as an important determinant of stability of the complex. We propose that the FTZ-F1 helix along with the core DBD serves as a platform for interactions with coactivators and other DNA-bound factors in the vicinity.

==About this Structure==

2FF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].

2FF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].

==Reference==

Line 13:

[[Category: Mus musculus]]

[[Category: Single protein]]

[[Category: Little, T.H.]]

[[Category: Little, T H.]]

[[Category: Matulis, C.K.]]

[[Category: Matulis, C K.]]

[[Category: Mayo, K.E.]]

[[Category: Mayo, K E.]]

[[Category: Radhakrishnan, I.]]

[[Category: Ramachandran, A.]]

Line 26:

[[Category: protein-dna complex]]

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@@ Line 1: / Line 1: @@
-[[Image:2ff0.gif|left|200px]]<br /><applet load="2ff0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ff0.gif|left|200px]]<br /><applet load="2ff0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2ff0" />
 '''Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter'''<br />
 ==Overview==
-Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear, hormone receptors and is considered a master regulator of reproduction, because it regulates a number of genes encoding reproductive hormones and, enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the, FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all, nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we, describe the solution structure of the SF1 DBD in complex with an atypical, sequence in the proximal promoter region of the inhibin-alpha gene that, encodes a subunit of a reproductive hormone. SF1 forms a specific complex, with the DNA through a bipartite motif binding to the major and minor, grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric, receptors, nerve growth factor-induced-B and estrogen-related receptor 2., However, unlike these receptors, SF1 harbors a helix in the C-terminal, segment of the FTZ-F1 box that interacts with both the core DBD and DNA, and serves as an important determinant of stability of the complex. We, propose that the FTZ-F1 helix along with the core DBD serves as a platform, for interactions with coactivators and other DNA-bound factors in the, vicinity.
+Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear hormone receptors and is considered a master regulator of reproduction because it regulates a number of genes encoding reproductive hormones and enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-alpha gene that encodes a subunit of a reproductive hormone. SF1 forms a specific complex with the DNA through a bipartite motif binding to the major and minor grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric receptors, nerve growth factor-induced-B and estrogen-related receptor 2. However, unlike these receptors, SF1 harbors a helix in the C-terminal segment of the FTZ-F1 box that interacts with both the core DBD and DNA and serves as an important determinant of stability of the complex. We propose that the FTZ-F1 helix along with the core DBD serves as a platform for interactions with coactivators and other DNA-bound factors in the vicinity.
 ==About this Structure==
-FF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].
+FF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Mus musculus]]
 [[Category: Single protein]]
-[[Category: Little, T.H.]]
+[[Category: Little, T H.]]
-[[Category: Matulis, C.K.]]
+[[Category: Matulis, C K.]]
-[[Category: Mayo, K.E.]]
+[[Category: Mayo, K E.]]
 [[Category: Radhakrishnan, I.]]
 [[Category: Ramachandran, A.]]
@@ Line 26: / Line 26: @@
 [[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:31:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:43 2008''