2fil: Difference between revisions

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==Overview==

2'-Deoxy-2'-fluoro-arabinonucleic acid (FANA) and arabinonucleic acid, (ANA) paired to RNA are substrates of RNase H. The conformation of the, natural DNA/RNA hybrid substrates appears to be neither A-form nor B-form., Consistent with this, the conformations of FANA and ANA were found to be, intermediate between the A- and B-forms. However, FANA opposite RNA is, preferred by RNase H over ANA, and the RNA affinity of FANA considerably, exceeds that of ANA. By investigating the conformational boundaries of, FANA and ANA residues in crystal structures of A- and B-form DNA duplexes, at atomic resolution, we demonstrate that FANA and ANA display subtle, conformational differences. The structural data provide insight into the, structural requirements at the catalytic site of RNase H. They also allow, conclusions with regard to the relative importance of stereoelectronic, effects and hydration as modulators of RNA affinity.

2'-Deoxy-2'-fluoro-arabinonucleic acid (FANA) and arabinonucleic acid (ANA) paired to RNA are substrates of RNase H. The conformation of the natural DNA/RNA hybrid substrates appears to be neither A-form nor B-form. Consistent with this, the conformations of FANA and ANA were found to be intermediate between the A- and B-forms. However, FANA opposite RNA is preferred by RNase H over ANA, and the RNA affinity of FANA considerably exceeds that of ANA. By investigating the conformational boundaries of FANA and ANA residues in crystal structures of A- and B-form DNA duplexes at atomic resolution, we demonstrate that FANA and ANA display subtle conformational differences. The structural data provide insight into the structural requirements at the catalytic site of RNase H. They also allow conclusions with regard to the relative importance of stereoelectronic effects and hydration as modulators of RNA affinity.

==About this Structure==

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[[Category: sugar modifications]]

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 ==Overview==
-'-Deoxy-2'-fluoro-arabinonucleic acid (FANA) and arabinonucleic acid, (ANA) paired to RNA are substrates of RNase H. The conformation of the, natural DNA/RNA hybrid substrates appears to be neither A-form nor B-form., Consistent with this, the conformations of FANA and ANA were found to be, intermediate between the A- and B-forms. However, FANA opposite RNA is, preferred by RNase H over ANA, and the RNA affinity of FANA considerably, exceeds that of ANA. By investigating the conformational boundaries of, FANA and ANA residues in crystal structures of A- and B-form DNA duplexes, at atomic resolution, we demonstrate that FANA and ANA display subtle, conformational differences. The structural data provide insight into the, structural requirements at the catalytic site of RNase H. They also allow, conclusions with regard to the relative importance of stereoelectronic, effects and hydration as modulators of RNA affinity.
+'-Deoxy-2'-fluoro-arabinonucleic acid (FANA) and arabinonucleic acid (ANA) paired to RNA are substrates of RNase H. The conformation of the natural DNA/RNA hybrid substrates appears to be neither A-form nor B-form. Consistent with this, the conformations of FANA and ANA were found to be intermediate between the A- and B-forms. However, FANA opposite RNA is preferred by RNase H over ANA, and the RNA affinity of FANA considerably exceeds that of ANA. By investigating the conformational boundaries of FANA and ANA residues in crystal structures of A- and B-form DNA duplexes at atomic resolution, we demonstrate that FANA and ANA display subtle conformational differences. The structural data provide insight into the structural requirements at the catalytic site of RNase H. They also allow conclusions with regard to the relative importance of stereoelectronic effects and hydration as modulators of RNA affinity.
 ==About this Structure==
@@ Line 19: / Line 19: @@
 [[Category: sugar modifications]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:37:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:21:45 2008''