2fs2: Difference between revisions
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New page: left|200px<br /><applet load="2fs2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fs2, resolution 2.000Å" /> '''Structure of the E.... |
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[[Image:2fs2.gif|left|200px]]<br /><applet load="2fs2" size=" | [[Image:2fs2.gif|left|200px]]<br /><applet load="2fs2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2fs2, resolution 2.000Å" /> | caption="2fs2, resolution 2.000Å" /> | ||
'''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon'''<br /> | '''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon'''<br /> | ||
==Overview== | ==Overview== | ||
The structure and biochemical function of the hot dog-fold thioesterase | The structure and biochemical function of the hot dog-fold thioesterase PaaI operative in the aerobic phenylacetate degradation pathway are examined. PaaI showed modest activity with phenylacetyl-coenzyme A, suggestive of a role in coenzyme A release from this pathway intermediate in the event of limiting downstream pathway enzymes. Minimal activity was observed with aliphatic acyl-coenzyme A thioesters, which ruled out PaaI function in the lower phenylacetate pathway. PaaI was most active with ring-hydroxylated phenylacetyl-coenzyme A thioesters. The x-ray crystal structure of the Escherichia coli thioesterase is reported and analyzed to define the structural basis of substrate recognition and catalysis. The contributions of catalytic and substrate binding residues, thus, identified were examined through steady-state kinetic analysis of site-directed mutant proteins. | ||
==About this Structure== | ==About this Structure== | ||
2FS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2FS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Buglino, J | [[Category: Buglino, J A.]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: Kniewel, R.]] | [[Category: Kniewel, R.]] | ||
[[Category: Lima, C | [[Category: Lima, C D.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Solorzano, V.]] | [[Category: Solorzano, V.]] | ||
[[Category: Wu, J.]] | [[Category: Wu, J.]] | ||
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[[Category: t820]] | [[Category: t820]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:31 2008'' | ||
