2ftu: Difference between revisions

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[[Image:2ftu.gif|left|200px]]<br />

[[Image:2ftu.gif|left|200px]]<br /><applet load="2ftu" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2ftu" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2ftu" />

'''solution structure of domain 3 of RAP'''<br />

==Overview==

The receptor associated protein (RAP) is an antagonist and molecular, chaperone that binds tightly to low-density lipoprotein receptor family, members in the endoplasmic reticulum (ER). After escorting these receptors, to the Golgi, RAP dissociates from the receptors. The molecular mechanism, of the dissociation has been unknown until now. The solution structure of, RAP-D3 domain presented here reveals a striking increase in positively, charged residues on the surface of this RAP domain due to protonation of, solvent-exposed histidine sidechains as the pH is reduced from a near, neutral pH of the ER to the acidic pH of the Golgi. Structure-based, mutagenesis studies in vitro and in cells confirm that the protonation of, histidine residues as a consequence of the pH changes modulate the, binding/release of RAP from LRP. This histidine switch may serve as a, general mechanism for regulating cell trafficking events.

The receptor associated protein (RAP) is an antagonist and molecular chaperone that binds tightly to low-density lipoprotein receptor family members in the endoplasmic reticulum (ER). After escorting these receptors to the Golgi, RAP dissociates from the receptors. The molecular mechanism of the dissociation has been unknown until now. The solution structure of RAP-D3 domain presented here reveals a striking increase in positively charged residues on the surface of this RAP domain due to protonation of solvent-exposed histidine sidechains as the pH is reduced from a near neutral pH of the ER to the acidic pH of the Golgi. Structure-based mutagenesis studies in vitro and in cells confirm that the protonation of histidine residues as a consequence of the pH changes modulate the binding/release of RAP from LRP. This histidine switch may serve as a general mechanism for regulating cell trafficking events.

==About this Structure==

2FTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2FTU OCA].

2FTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FTU OCA].

==Reference==

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Line 14:

[[Category: Single protein]]

[[Category: Lee, D.]]

[[Category: Walsh, J.D.]]

[[Category: Walsh, J D.]]

[[Category: Wang, Y.X.]]

[[Category: Wang, Y X.]]

[[Category: domain 3; rap; receptor-associated protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 22~~:10:~~16 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:03 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2ftu.gif|left|200px]]<br />
+[[Image:2ftu.gif|left|200px]]<br /><applet load="2ftu" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2ftu" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2ftu" />
 '''solution structure of domain 3 of RAP'''<br />
 ==Overview==
-The receptor associated protein (RAP) is an antagonist and molecular, chaperone that binds tightly to low-density lipoprotein receptor family, members in the endoplasmic reticulum (ER). After escorting these receptors, to the Golgi, RAP dissociates from the receptors. The molecular mechanism, of the dissociation has been unknown until now. The solution structure of, RAP-D3 domain presented here reveals a striking increase in positively, charged residues on the surface of this RAP domain due to protonation of, solvent-exposed histidine sidechains as the pH is reduced from a near, neutral pH of the ER to the acidic pH of the Golgi. Structure-based, mutagenesis studies in vitro and in cells confirm that the protonation of, histidine residues as a consequence of the pH changes modulate the, binding/release of RAP from LRP. This histidine switch may serve as a, general mechanism for regulating cell trafficking events.
+The receptor associated protein (RAP) is an antagonist and molecular chaperone that binds tightly to low-density lipoprotein receptor family members in the endoplasmic reticulum (ER). After escorting these receptors to the Golgi, RAP dissociates from the receptors. The molecular mechanism of the dissociation has been unknown until now. The solution structure of RAP-D3 domain presented here reveals a striking increase in positively charged residues on the surface of this RAP domain due to protonation of solvent-exposed histidine sidechains as the pH is reduced from a near neutral pH of the ER to the acidic pH of the Golgi. Structure-based mutagenesis studies in vitro and in cells confirm that the protonation of histidine residues as a consequence of the pH changes modulate the binding/release of RAP from LRP. This histidine switch may serve as a general mechanism for regulating cell trafficking events.
 ==About this Structure==
-FTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FTU OCA].
+FTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FTU OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Lee, D.]]
-[[Category: Walsh, J.D.]]
+[[Category: Walsh, J D.]]
-[[Category: Wang, Y.X.]]
+[[Category: Wang, Y X.]]
 [[Category: domain 3; rap; receptor-associated protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:10:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:03 2008''