2fx3: Difference between revisions

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[[Image:2fx3.gif|left|200px]]<br /><applet load="2fx3" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2fx3.gif|left|200px]]<br /><applet load="2fx3" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2fx3, resolution 3.400Å" />

'''Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set'''<br />

==Overview==

Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in, the presence of novel inhibitors. The only crystals which could be grown, were epitaxially as well as merohedrally twinned, highly mosaic and, diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell, parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120, degrees . To determine whether an inhibitor was present in the crystal, a, poor-quality X-ray diffraction data set had to be processed. The, three-dimensional structure was ultimately solved and the original, question answered. The results also reveal a new type of dimer packing for, EF-Tu-GDP.

Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.

==About this Structure==

2FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].

2FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Heffron, S.E.]]

[[Category: Heffron, S E.]]

[[Category: Jurnak, F.]]

[[Category: Moeller, R.]]

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[[Category: merohedral twinning]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 10:49:~~13 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:56 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2fx3.gif|left|200px]]<br /><applet load="2fx3" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fx3.gif|left|200px]]<br /><applet load="2fx3" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2fx3, resolution 3.400&Aring;" />
 '''Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set'''<br />
 ==Overview==
-Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in, the presence of novel inhibitors. The only crystals which could be grown, were epitaxially as well as merohedrally twinned, highly mosaic and, diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell, parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120, degrees . To determine whether an inhibitor was present in the crystal, a, poor-quality X-ray diffraction data set had to be processed. The, three-dimensional structure was ultimately solved and the original, question answered. The results also reveal a new type of dimer packing for, EF-Tu-GDP.
+Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.
 ==About this Structure==
-FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].
+FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Heffron, S.E.]]
+[[Category: Heffron, S E.]]
 [[Category: Jurnak, F.]]
 [[Category: Moeller, R.]]
@@ Line 21: / Line 21: @@
 [[Category: merohedral twinning]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:49:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:56 2008''