2hit: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="2hit" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hit, resolution 2.75Å" /> '''Reaction centre from... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2hit.gif|left|200px]]<br /><applet load="2hit" size=" | [[Image:2hit.gif|left|200px]]<br /><applet load="2hit" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2hit, resolution 2.75Å" /> | caption="2hit, resolution 2.75Å" /> | ||
'''Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine'''<br /> | '''Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine'''<br /> | ||
==Overview== | ==Overview== | ||
This study describes the use of brominated phospholipids to distinguish | This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins. | ||
==About this Structure== | ==About this Structure== | ||
2HIT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE, K, CL, PO4, BCL, BPH, U10, CDL, PEV, PEW, LDA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2HIT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=BCL:'>BCL</scene>, <scene name='pdbligand=BPH:'>BPH</scene>, <scene name='pdbligand=U10:'>U10</scene>, <scene name='pdbligand=CDL:'>CDL</scene>, <scene name='pdbligand=PEV:'>PEV</scene>, <scene name='pdbligand=PEW:'>PEW</scene>, <scene name='pdbligand=LDA:'>LDA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIT OCA]. | ||
==Reference== | ==Reference== | ||
Brominated | Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides., Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ, Biochemistry. 2007 Mar 20;46(11):2909-16. Epub 2007 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17315985 17315985] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
[[Category: Cogdell, R | [[Category: Cogdell, R J.]] | ||
[[Category: Gardiner, A | [[Category: Gardiner, A T.]] | ||
[[Category: Isaacs, N | [[Category: Isaacs, N W.]] | ||
[[Category: Roszak, A | [[Category: Roszak, A W.]] | ||
[[Category: BCL]] | [[Category: BCL]] | ||
[[Category: BPH]] | [[Category: BPH]] | ||
| Line 35: | Line 35: | ||
[[Category: photosynthetic reaction center]] | [[Category: photosynthetic reaction center]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:42:22 2008'' | ||
