2hmf: Difference between revisions
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New page: left|200px<br /><applet load="2hmf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hmf, resolution 2.700Å" /> '''Structure of a Thre... |
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[[Image:2hmf.gif|left|200px]]<br /><applet load="2hmf" size=" | [[Image:2hmf.gif|left|200px]]<br /><applet load="2hmf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2hmf, resolution 2.700Å" /> | caption="2hmf, resolution 2.700Å" /> | ||
'''Structure of a Threonine Sensitive Aspartokinase from Methanococcus jannaschii Complexed with Mg-ADP and Aspartate'''<br /> | '''Structure of a Threonine Sensitive Aspartokinase from Methanococcus jannaschii Complexed with Mg-ADP and Aspartate'''<br /> | ||
==Overview== | ==Overview== | ||
The activation of the beta-carboxyl group of aspartate catalyzed by | The activation of the beta-carboxyl group of aspartate catalyzed by aspartokinase is the commitment step to amino-acid biosynthesis in the aspartate pathway. The first structure of a microbial aspartokinase, that from Methanococcus jannaschii, has been determined in the presence of the amino-acid substrate L-aspartic acid and the nucleotide product MgADP. The enzyme assembles into a dimer of dimers, with the interfaces mediated by both the N- and C-terminal domains. The active-site functional groups responsible for substrate binding and specificity have been identified and roles have been proposed for putative catalytic functional groups. | ||
==About this Structure== | ==About this Structure== | ||
2HMF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MG, ADP and ASP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] Full crystallographic information is available from [http:// | 2HMF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=ASP:'>ASP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMF OCA]. | ||
==Reference== | ==Reference== | ||
The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinase., Faehnle CR, Liu X, Pavlovsky A, Viola RE, Acta | The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinase., Faehnle CR, Liu X, Pavlovsky A, Viola RE, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):962-6. Epub 2006 Sep 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17012784 17012784] | ||
[[Category: Aspartate kinase]] | [[Category: Aspartate kinase]] | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Faehnle, C | [[Category: Faehnle, C R.]] | ||
[[Category: Viola, R | [[Category: Viola, R E.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: ASP]] | [[Category: ASP]] | ||
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[[Category: aspartokinase]] | [[Category: aspartokinase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:26 2008'' | ||
